FMG-1B #8
Catalog Fields
Antigen: Flagellar membrane glycoprotein 1B/ FMG-1B
Hybridoma Cells Available: Yes
Antigen Species: Chlamydomonas
Depositor: Bloodgood, R.A.
Isotype: MIgG1
Depositors Institution: University of Virginia
Host Species: mouse
Depositors Notes: FMG-1B #8 binds to a carbohydrate epitope.
Positive Tested Species Reactivity: Chlamydomonas
Gene: FMG-1B
Antigen Molecular Weight: 350 kDa apparent
Clonality: Monoclonal
Immunogen: Detergent-extracted denatured flagellar proteins
Epitope Mapped: No
Myeloma Strain: SP2/0
Immunogen Sequence: GenBank: AAO25118.2
Antibody Registry ID: AB_2722111
Additional Information: Bloodgood, R.A. (2009) The Chlamydomonas flagellar membrane and its dynamic properties. IN:
Chlamydomonas in Motility Research (Ed. G.W. Witman and E. Harris). Vol. III of The Chlamydomonas Sourcebook. New
York, Academic Press, 309?368. RRID:AB_2722111
Chlamydomonas in Motility Research (Ed. G.W. Witman and E. Harris). Vol. III of The Chlamydomonas Sourcebook. New
York, Academic Press, 309?368. RRID:AB_2722111
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
FMG-1B #8 was deposited to the DSHB by Bloodgood, R.A. (DSHB Hybridoma Product FMG-1B #8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Redistribution and shedding of flagellar membrane glycoproteins visualized using an anti-carbohydrate monoclonal antibody and concanavalin A.
Salomonsky NL
The Journal of cell biology 102.5 (1986 May): 1797-812.