GBB 3D6-24

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SKU: GBB 3D6-24-s
View product citations for antibody GBB 3D6-24 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Glass bottom boat; Protein 60A
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Marques, G.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Minnesota
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Gbb exists in two ligand forms: 15 kDa and 38 kDa.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: gbb
Immunogen: Recombinant protein
Alternate Gene Names: 60A; BMP; CG5562; Dm-GBB; TGF-b; TGFbeta-60A; vgr/60A
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63-Ag8.653,P3X63-Ag8
Epitope Location or Sequence: C-terminal a.a 424-440
Uniprot ID: P27091 
Immunogen Sequence: C-terminal a.a. 426-440
Entrez Gene ID: 37778 
Additional Characterization:  
Antibody Registry ID: AB_2618111 
Additional Information: RRID:AB_2618111
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GBB 3D6-24 was deposited to the DSHB by Marques, G. (DSHB Hybridoma Product GBB 3D6-24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    WB References
    IHC References
    IP References
    All References

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