GBB 3D6-24

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SKU: GBB 3D6-24-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: GBB 3D6-24
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: gbb
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2618111 
Uniprot ID: P27091 
RRID:  
Entrez Gene ID: 37778 
Clonality: Monoclonal
Immunogen: Recombinant protein
Clone:
Immunogen Sequence: C-terminal a.a. 426-440
Myeloma Strain: P3X63-Ag8.653,P3X63-Ag8
Epitope Mapped: Yes
Antigen Name: Glass bottom boat; Protein 60A
Epitope Location or Sequence: C-terminal a.a 424-440
Alternate Antigen Name:
Deposit Date: 7/17/2012
Antigen Molecular Weight: Gbb exists in two ligand forms: 15 kDa and 38 kDa.
Depositor: Marques, G.
Antigen Sequence:
Depositor Institution: University of Minnesota
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_2618111
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GBB 3D6-24 was deposited to the DSHB by Marques, G. (DSHB Hybridoma Product GBB 3D6-24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    WB References
    IHC References
    IP References
    All References

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