GBB 3D6-24

(0)No Reviews yet
SKU: GBB 3D6-24-s
View product citations for antibody GBB 3D6-24 on CiteAb

In Stock

Available: 78

DSHB Data Sheet

Catalog Fields

Antigen: Glass bottom boat; Protein 60A
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG2b
Depositor: Marques, G.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of Minnesota
Antigen Molecular Weight: Gbb exists in two ligand forms: 15 kDa and 38 kDa.
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant protein
Gene: gbb
Alternate Antibody Name:
Alternate Gene Names: 60A; BMP; CG5562; Dm-GBB; TGF-b; TGFbeta-60A; vgr/60A
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63-Ag8.653,P3X63-Ag8
Epitope Mapped: Yes
Uniprot ID: P27091 
Epitope Location or Sequence: C-terminal a.a 424-440
Entrez Gene ID: 37778 
Immunogen Sequence: C-terminal a.a. 426-440
Antibody Registry ID: AB_2618111 
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: RRID:AB_2618111
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GBB 3D6-24 was deposited to the DSHB by Marques, G. (DSHB Hybridoma Product GBB 3D6-24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    WB References
    IHC References
    IP References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review