HA4c19
Catalog Fields
Antigen: Carcinoembryonic antigen-related cell adhesion molecule 1; Insulin receptor kinase substrate pp120; CD66a;
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Hubbard, A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University, Baltimore
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: This antibody is a cell marker for hepatocyte bile canalicular epithelial cells and is also known as billiary glycoprotein.
Predicted Species Reactivity:
Gene: Ceacam1
Immunogen: Rat hepatocyte membranes
Alternate Gene Names: BGPR, Bgp, CD66a, Ccam1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8UI
Epitope Location or Sequence:
Uniprot ID: P16573
Immunogen Sequence: Full length protein
Entrez Gene ID: 81613
Antibody Registry ID: AB_10659875
Additional Information: RRID:AB_10659875
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HA4c19 was deposited to the DSHB by Hubbard, A. (DSHB Hybridoma Product HA4c19)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies.
Braiterman LT
The Journal of cell biology 100.4 (1985 Apr): 1115-25.