Catalog Fields
Antigen: Carcinoembryonic antigen-related cell adhesion molecule 1; Insulin receptor kinase substrate pp120; CD66a;
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Hubbard, A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Mouse, Rat
Depositors Institution: Johns Hopkins University
Antigen Molecular Weight: 110-115 kDa
Depositors Notes: This antibody is a cell marker for hepatocyte bile canalicular epithelial cells and is also known as billiary glycoprotein.
Immunogen: Rat hepatocyte membranes
Gene: Ceacam1
Alternate Antibody Name:
Alternate Gene Names: BGPR, Bgp, CD66a, Ccam1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8UI
Epitope Mapped: No
Uniprot ID: P16573
Epitope Location or Sequence:
Entrez Gene ID: 81613
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_10659875
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: RRID:AB_10659875
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HA4c19 was deposited to the DSHB by Hubbard, A. (DSHB Hybridoma Product HA4c19)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
9 References
Initial Publication
Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies.
Braiterman LT
The Journal of cell biology 100.4 (1985 Apr): 1115-25.