HA4c19

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$40.00
SKU: HA4c19
View product citations for antibody HA4c19 on CiteAb

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Available: 53

DSHB Data Sheet

Catalog Fields

Antigen: Carcinoembryonic antigen-related cell adhesion molecule 1; Insulin receptor kinase substrate pp120; CD66a;
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Hubbard, A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University, Baltimore
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: This antibody is a cell marker for hepatocyte bile canalicular epithelial cells and is also known as billiary glycoprotein.
Antigen Molecular Weight: 110-115 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ceacam1
Immunogen: Rat hepatocyte membranes
Alternate Gene Names: BGPR, Bgp, CD66a, Ccam1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8UI
Epitope Location or Sequence:
Uniprot ID: P16573 
Immunogen Sequence: Full length protein
Entrez Gene ID: 81613 
Additional Characterization:  
Antibody Registry ID: AB_10659875 
Additional Information: RRID:AB_10659875
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HA4c19 was deposited to the DSHB by Hubbard, A. (DSHB Hybridoma Product HA4c19)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies.
    Braiterman LT
    The Journal of cell biology 100.4 (1985 Apr): 1115-25.

    IF References

    Insulin stimulates pp120 endocytosis in cells co-expressing insulin receptors.
    Najjar SM
    The Journal of biological chemistry 273.35 (1998 Aug 28): 22194-200.

    Insulin acutely decreases hepatic fatty acid synthase activity.
    Oyarce AM
    Cell metabolism 2.1 (2005 Jul): 43-53.

    Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the bile canaliculus.
    Sandoval IV
    Journal of cell science 129.11 (2016 Jun 1): 2190-201.

    Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies.
    Braiterman LT
    The Journal of cell biology 100.4 (1985 Apr): 1115-25.

    WB References

    Identification of pp120, an endogenous substrate for the hepatocyte insulin receptor tyrosine kinase, as an integral membrane glycoprotein of the bile canalicular domain.
    Hubbard AL
    Proceedings of the National Academy of Sciences of the United States of America 85.19 (1988 Oct): 7256-9.

    Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion.
    Keyel PA
    Frontiers in immunology 8. (2017): 522.

    Effect of pp120 on receptor-mediated insulin endocytosis is regulated by the juxtamembrane domain of the insulin receptor.
    Poy MN
    The Journal of biological chemistry 273.21 (1998 May 22): 12923-8.

    Insulin acutely decreases hepatic fatty acid synthase activity.
    Oyarce AM
    Cell metabolism 2.1 (2005 Jul): 43-53.

    Endogenous and exogenous domain markers of the rat hepatocyte plasma membrane.
    Hubbard AL
    The Journal of cell biology 100.4 (1985 Apr): 1126-38.

    IP References
    All References

    Insulin stimulates pp120 endocytosis in cells co-expressing insulin receptors.
    Najjar SM
    The Journal of biological chemistry 273.35 (1998 Aug 28): 22194-200.

    Insulin acutely decreases hepatic fatty acid synthase activity.
    Oyarce AM
    Cell metabolism 2.1 (2005 Jul): 43-53.

    Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the bile canaliculus.
    Sandoval IV
    Journal of cell science 129.11 (2016 Jun 1): 2190-201.

    Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies.
    Braiterman LT
    The Journal of cell biology 100.4 (1985 Apr): 1115-25.

    Identification of pp120, an endogenous substrate for the hepatocyte insulin receptor tyrosine kinase, as an integral membrane glycoprotein of the bile canalicular domain.
    Hubbard AL
    Proceedings of the National Academy of Sciences of the United States of America 85.19 (1988 Oct): 7256-9.

    Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion.
    Keyel PA
    Frontiers in immunology 8. (2017): 522.

    Effect of pp120 on receptor-mediated insulin endocytosis is regulated by the juxtamembrane domain of the insulin receptor.
    Poy MN
    The Journal of biological chemistry 273.21 (1998 May 22): 12923-8.

    Endogenous and exogenous domain markers of the rat hepatocyte plasma membrane.
    Hubbard AL
    The Journal of cell biology 100.4 (1985 Apr): 1126-38.

    The differential effects of pp120 (Ceacam 1) on the mitogenic action of insulin and insulin-like growth factor 1 are regulated by the nonconserved tyrosine 1316 in the insulin receptor.
    Najjar SM
    Molecular and cellular biology 20.11 (2000 Jun): 3896-905.

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