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SKU: HBB3/153/63
View product citations for antibody HBB3/153/63 on CiteAb

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Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: aminopeptidase N (CD13)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Hauri, H.-P.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Basel
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as alanyl aminopeptidase.
Antigen Molecular Weight: 162 kDa (apparent)
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: Purified intestinal microvillus membranes
Alternate Gene Names: APN, CD13, PEPN
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: FO
Epitope Location or Sequence:
Uniprot ID: P15144 
Immunogen Sequence: Full length protein
Entrez Gene ID: 290 
Additional Characterization:  
Antibody Registry ID: AB_2258367 
Additional Information: RRID: AB_2258367
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HBB3/153/63 was deposited to the DSHB by Hauri, H.-P. (DSHB Hybridoma Product HBB3/153/63)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • All References
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