HCS-1

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$40.00
SKU: HCS-1
View product citations for antibody HCS-1 on CiteAb

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Available: 173

DSHB Data Sheet

Catalog Fields

Antigen: Otoferlin; Hair cell soma-1
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Corwin, J.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Amphibian, Avian, Elasmobranch, Fish, Mammal, Reptile
Depositors Notes: This antigen is a key calcium ion sensor regulating neurotransmitter release. The antigen is also known as Fer-1-like protein 2.
Antigen Molecular Weight: 210 kDa /230 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: OTOF
Immunogen: Delaminated chick utricles emulsion
Alternate Gene Names: DFNB6, DFNB9, FER1L2
Alternate Antibody Name: otoferlin
Clonality: Monoclonal
Alternate Antigen Name: otoferlin
Epitope Mapped:
Myeloma Strain: SP/0
Epitope Location or Sequence:
Uniprot ID: Q9HC10 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 9381 
Additional Characterization:
Antibody Registry ID: AB_10804296 
Additional Information: RRID:AB_10804296
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HCS-1 was deposited to the DSHB by Corwin, J. (DSHB Hybridoma Product HCS-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    IF References

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    Expression and Misexpression of the miR-183 Family in the Developing Hearing Organ of the Chicken.
    Fekete DM
    PloS one 10.7 (2015): e0132796.

    Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance.
    Perkins BD
    Scientific reports 9.1 (2019 Jul 16): 10302.

    IHC References

    Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish.
    Lecaudey V
    eLife 4. (2015 Sep 3): e08201.

    Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea.
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 37.37 (2017 Sep 13): 8975-8988.

    IP References

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    All References

    Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish.
    Lecaudey V
    eLife 4. (2015 Sep 3): e08201.

    Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea.
    Fekete DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 37.37 (2017 Sep 13): 8975-8988.

    Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
    Richardson GP
    Journal of the Association for Research in Otolaryngology : JARO 11.4 (2010 Dec): 573-86.

    Expression and Misexpression of the miR-183 Family in the Developing Hearing Organ of the Chicken.
    Fekete DM
    PloS one 10.7 (2015): e0132796.

    Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance.
    Perkins BD
    Scientific reports 9.1 (2019 Jul 16): 10302.

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