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SKU: Hao3
View product citations for antibody Hao3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: dE2F1, Transcription factor
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Dyson, N.J.
Isotype: MIgG1
Depositors Institution: Harvard Medical School
Host Species: mouse
Gene: E2f1
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: E2f, CG6376
Antigen Molecular Weight: 87.4 kDa
Clonality: Monoclonal
Immunogen: Recombinant dE2F protein
Epitope Mapped: No
Myeloma Strain: NS-1
Immunogen Sequence: full-length
Uniprot ID: Q27368 
Entrez Gene ID: 42550 
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Hao3 was deposited to the DSHB by Dyson, N.J. (DSHB Hybridoma Product Hao3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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