IAD 6-2

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SKU: IAD 6-2-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: IAD 6-2
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: IGHD
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2796590 
Uniprot ID: P01880 
RRID:  
Entrez Gene ID: 3495 
Clonality: Monoclonal
Immunogen: IgD myeloma protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: P3X63.Ag8.653
Epitope Mapped: No
Antigen Name: Human IgD
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 184 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Human
Depositor Notes: Hybridoma developed by J.F.Kearney.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IAD 6-2 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product IAD 6-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

5 References

  • Initial Publication
  • IHC References
  • IP References
  • FFPE References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells.
    Kubagawa H
    Journal of immunology (Baltimore, Md. : 1950) 138.12 (1987 Jun 15): 4150-8.

    IHC References

    IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Lipsky PE
    Journal of immunology (Baltimore, Md. : 1950) 178.5 (2007 Mar 1): 2872-82.

    [Protective work respiration masks and the bacterial flora of upper respiratory pathways].
    Chládek V
    Ceskoslovenska otolaryngologie 27.5 (1978 Oct): 274-7.

    IP References

    Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells.
    Kubagawa H
    Journal of immunology (Baltimore, Md. : 1950) 138.12 (1987 Jun 15): 4150-8.

    FFPE References

    [Protective work respiration masks and the bacterial flora of upper respiratory pathways].
    Chládek V
    Ceskoslovenska otolaryngologie 27.5 (1978 Oct): 274-7.

    FB References
    FACS References
    All References

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