IFT139.1
Catalog Fields
Clone ID/Product Name: IFT139.1
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: IFT139
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_2619555
Uniprot ID: A9XPA6
Entrez Gene ID: 175855
Clonality: Monoclonal
Immunogen: Purified intraflagellar transport complexes A and B
Clone:
Immunogen Sequence:
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Intraflagellar transport protein p139
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/29/2015
Antigen Molecular Weight: apparent 139 kDa; actual 152 kDa
Depositor: Rosenbaum, J.L.
Antigen Sequence:
Depositor Institution: Yale University
Antigen Species: Chlamydomonas
Depositor Notes: This antibody is a marker for IFT complex A.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chlamydomonas
Additional Information: RRID:AB_2619555
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IFT139.1 was deposited to the DSHB by Rosenbaum, J.L. (DSHB Hybridoma Product IFT139.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
4 References
Initial Publication
Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.
Rosenbaum JL
The Journal of cell biology 141.4 (1998 May 18): 993-1008.