IFT139.1
Catalog Fields
Antigen: Intraflagellar transport protein p139
Hybridoma Cells Available: Yes
Antigen Species: Chlamydomonas
Depositor: Rosenbaum, J.L.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University
Positive Tested Species Reactivity: Chlamydomonas
Depositors Notes: This antibody is a marker for IFT complex A.
Antigen Molecular Weight: apparent 139 kDa; actual 152 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: IFT139
Immunogen: Purified intraflagellar transport complexes A and B
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID: A9XPA6
Immunogen Sequence:
Entrez Gene ID: 175855
Additional Characterization:
Antibody Registry ID: AB_2619555
Additional Information: RRID:AB_2619555
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IFT139.1 was deposited to the DSHB by Rosenbaum, J.L. (DSHB Hybridoma Product IFT139.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.
Rosenbaum JL
The Journal of cell biology 141.4 (1998 May 18): 993-1008.