IFT57.1

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$40.00
SKU: IFT57.1
View product citations for antibody IFT57.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Intraflagellar transport protein p57/55
Hybridoma Cells Available: Yes
Antigen Species: Chlamydomonas
Depositor: Rosenbaum, J.L.
Isotype: MIgG1
Depositors Institution: Yale University
Host Species: mouse
Gene: IFT57
Positive Tested Species Reactivity: Chlamydomonas
Clonality: Monoclonal
Antigen Molecular Weight: apparent 57/55 kDa; actual 51 kDa
Epitope Mapped: No
Immunogen: Purified intraflagellar transport complexes A and B
Additional Information: RRID:AB_2619557
Myeloma Strain: Sp2/0
Uniprot ID: Q2XQY7 
Entrez Gene ID: 5724201 
Antibody Registry ID: AB_2619557 
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IFT57.1 was deposited to the DSHB by Rosenbaum, J.L. (DSHB Hybridoma Product IFT57.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

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