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SKU: IFT81.4
View product citations for antibody IFT81.4 on CiteAb

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Available: 41

DSHB Data Sheet

Catalog Fields

Antigen: Intraflagellar transport protein p81
Hybridoma Cells Available: Yes
Antigen Species: Chlamydomonas
Depositor: Rosenbaum, J.L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University
Positive Tested Species Reactivity: Chlamydomonas
Depositors Notes:
Antigen Molecular Weight: apparent 81 kDa; actual 77 kda
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: IFT81
Immunogen: Purified intraflagellar transport complexes A and B
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID: Q68RJ5 
Immunogen Sequence:
Entrez Gene ID: 5722735 
Additional Characterization:
Antibody Registry ID: AB_2619560 
Additional Information: RRID:AB_2619560
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IFT81.4 was deposited to the DSHB by Rosenbaum, J.L. (DSHB Hybridoma Product IFT81.4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    All References

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