JLJ5a

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$45.00 to $425.00
SKU: JLJ5a
View product citations for antibody JLJ5a on CiteAb

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Available: 13

DSHB Data Sheet

Catalog Fields

Antigen: Heat Shock Protein-100
Available to For-Profits: Yes
Antigen Species: Gerbil
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Lin, J.J.-C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chinese Hamster, Gerbil, Human, Rat, Tetrahymena
Depositors Institution: University of Iowa
Antigen Molecular Weight: 100 kDa
Depositors Notes: This antibody label a Golgi-associated heat shock protein which translocates to the nucleus under stress. This antibody also recognizes basal bodies in tetrahymena.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Gerbil IMR-33 cell lysate
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_2618102 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation
Additional Information: RRID:AB_2618102
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JLJ5a was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product JLJ5a)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • IP References
  • All References
  • Initial Publication

    A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    Queally SA
    The Journal of cell biology 92.1 (1982 Jan): 108-12.

    IF References

    A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    Queally SA
    The Journal of cell biology 92.1 (1982 Jan): 108-12.

    Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca2+-ionophore-regulated proteins.
    Feramisco JR
    The Journal of biological chemistry 258.11 (1983 Jun 10): 7102-11.

    Regional differentiation of the membrane skeleton in Tetrahymena.
    Jaeckel-Williams RF
    Journal of cell science 87 ( Pt 3). (1987 Apr): 457-63.

    IP References
    All References

    A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    Queally SA
    The Journal of cell biology 92.1 (1982 Jan): 108-12.

    Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca2+-ionophore-regulated proteins.
    Feramisco JR
    The Journal of biological chemistry 258.11 (1983 Jun 10): 7102-11.

    Regional differentiation of the membrane skeleton in Tetrahymena.
    Jaeckel-Williams RF
    Journal of cell science 87 ( Pt 3). (1987 Apr): 457-63.

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