JLJ5a

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$40.00
SKU: JLJ5a
View product citations for antibody JLJ5a on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Heat Shock Protein-100
Hybridoma Cells Available: Yes
Antigen Species: Gerbil
Depositor: Lin, J.J.-C.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Chinese Hamster, Gerbil, Human, Rat, Tetrahymena
Depositors Notes: This antibody label a Golgi-associated heat shock protein which translocates to the nucleus under stress. This antibody also recognizes basal bodies in tetrahymena.
Antigen Molecular Weight: 100 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Gerbil IMR-33 cell lysate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2618102 
Additional Information: RRID:AB_2618102
Recommended Applications: Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JLJ5a was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product JLJ5a)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IP References
  • All References
  • Initial Publication

    A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    Queally SA
    The Journal of cell biology 92.1 (1982 Jan): 108-12.

    IF References

    A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    Queally SA
    The Journal of cell biology 92.1 (1982 Jan): 108-12.

    Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca2+-ionophore-regulated proteins.
    Feramisco JR
    The Journal of biological chemistry 258.11 (1983 Jun 10): 7102-11.

    Regional differentiation of the membrane skeleton in Tetrahymena.
    Jaeckel-Williams RF
    Journal of cell science 87 ( Pt 3). (1987 Apr): 457-63.

    IP References
    All References

    A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
    Queally SA
    The Journal of cell biology 92.1 (1982 Jan): 108-12.

    Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca2+-ionophore-regulated proteins.
    Feramisco JR
    The Journal of biological chemistry 258.11 (1983 Jun 10): 7102-11.

    Regional differentiation of the membrane skeleton in Tetrahymena.
    Jaeckel-Williams RF
    Journal of cell science 87 ( Pt 3). (1987 Apr): 457-63.

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