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View product citations for antibody KLAR-N 9E1B on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: klarsicht, exon 4
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Wieschaus, E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes: mutant analysis indicates that it specifically recognizes Klar by western blotting. This antibody has not been used successfully for immunohistochemistry.
Antigen Molecular Weight: Predicted: multiple isoforms from 56 to 246 kDa; Apparent: major band above 250 kDa and multiple additional bands.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: klar
Immunogen: Recombinant GST/Klarsicht (isoform A, aa 277-556) fusion protein
Alternate Gene Names: CG17046, kls, marb
Alternate Antibody Name: KLAR-N
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 277-317 of isoform A
Uniprot ID: Q9W0Q4 
Immunogen Sequence: Partial protein
Entrez Gene ID: 38067 
Additional Characterization:  
Antibody Registry ID: AB_10586635 
Additional Information: KLAR-N should recognize isoforms A, B, D, and E (not C, F, G, H, or I) [isoform nomenclature from FlyBase].
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
KLAR-N 9E1B was deposited to the DSHB by Wieschaus, E. (DSHB Hybridoma Product KLAR-N 9E1B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    WB References
    Epitope Map References
    All References

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