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DSHB Data Sheet

Catalog Fields

Product Name/ID: M1BP
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: M1BP
Ab Isotype: Rabbit IgG
Gene Symbol:
Antibody Registry ID: AB_2938697 
Uniprot ID: Q9VHM3 
Entrez Gene ID: 38982 
Clonality: Polyclonal
Immunogen: His-tag fusion protein
Immunogen Sequence: Full-length
Myeloma Strain: N.A.
Epitope Mapped: No
Antigen Name: Motif 1 binding protein/M1BP
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/18/2023
Antigen Molecular Weight: 48.0 kDa
Depositor: Gilmour, D.S.
Antigen Sequence:
Depositor Institution: Penn State Univ.
Antigen Species: Drosophila
Depositor Notes: This antisera is good for immunofluorescence staining polytene chromosomes. An initial dilution of 1:200 is recommended.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: This antiserum is also validated for ChIP-seq and ChIP-exo.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization: https://flybase.org/reports/FBgn0037621 
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M1BP was deposited to the DSHB by Gilmour, D.S. (DSHB Hybridoma Product M1BP)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

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