MANDYS15(6B11)

(0) No Reviews yet
$40.00
SKU: MANDYS15(6B11)
View product citations for antibody MANDYS15(6B11) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin, exons 31/32
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK
Host Species: mouse
Depositors Notes: Doesn't work on Chicken or Xenopus. Fixation for immunostaining: acetone/methanol.
Positive Tested Species Reactivity: Canine, Human, Mouse
Gene: DMD
Antigen Molecular Weight: Predicted: 427 kDa; Apparent: 427 kDa
Alternate Gene Names: BMD, CMD3B, MRX85
Immunogen: Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Uniprot ID: P11532 
Epitope Location or Sequence: exons 31/32, aa 1431-1505
Entrez Gene ID: 1756 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2618165 
Recommended Applications: ELISA, Immunofluorescence, Western Blot
Additional Information: The collection of anti-DMD mAbs deposited by Morris, G.E., are directed against different DMD exons.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS15(6B11) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS15(6B11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin.
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies.
    Sewry CA
    Methods in molecular biology (Clifton, N.J.) 709. (2011): 39-61.

    Myoblast transfer in the treatment of Duchenne's muscular dystrophy.
    Rice R
    The New England journal of medicine 333.13 (1995 Sep 28): 832-8.

    WB References
    ELISA References
    Epitope Map References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review