MANDYS15(6B11)

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$40.00
SKU: MANDYS15(6B11)
View product citations for antibody MANDYS15(6B11) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin, exons 31/32
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Notes: Doesn't work on Chicken or Xenopus. Fixation for immunostaining: acetone/methanol.
Antigen Molecular Weight: Predicted: 427 kDa; Apparent: 427 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: DMD
Immunogen: Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein
Alternate Gene Names: BMD, CMD3B, MRX85
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: exons 31/32, aa 1431-1505
Uniprot ID: P11532 
Immunogen Sequence: Partial protein
Entrez Gene ID: 1756 
Antibody Registry ID: AB_2618165 
Additional Information: The collection of anti-DMD mAbs deposited by Morris, G.E., are directed against different DMD exons.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS15(6B11) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS15(6B11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin.
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies.
    Sewry CA
    Methods in molecular biology (Clifton, N.J.) 709. (2011): 39-61.

    Myoblast transfer in the treatment of Duchenne's muscular dystrophy.
    Rice R
    The New England journal of medicine 333.13 (1995 Sep 28): 832-8.

    WB References
    ELISA References
    Epitope Map References
    All References

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