MANNES2H(3C4)

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$40.00
SKU: MANNES2H(3C4)
View product citations for antibody MANNES2H(3C4) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Nesprin-2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as nuclear envelope spectrin repeat protein 2 or NUANCE.
Antigen Molecular Weight: 796.4 Kda
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SYNE2
Immunogen: Recombinant protein fragment
Alternate Gene Names: NUA
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q8WXH0 
Immunogen Sequence: Nt 19,831-20,853; C-terminal 38 kDa fragment
Entrez Gene ID: 23224 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANNES2H(3C4) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANNES2H(3C4))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    IF References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    WB References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    All References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

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