MANSMA20(8C1)

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$45.00 to $425.00
SKU: MANSMA20(8C1)
View product citations for antibody MANSMA20(8C1) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: SMN1 protein, exon 2
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Morris, G.E.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Molecular Weight: Predicted: 32 kDa; Apparent: 38 kDa
Depositors Notes: Stains only SMN band in HeLa total protein extract. Stains dot-like structures (gems) in the nucleus.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant SMN protein
Gene: SMN1
Alternate Antibody Name: MANSMA20
Alternate Gene Names: SMN, SMNT, SMN2, SMNC, Gemin1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Uniprot ID: Q16637 
Epitope Location or Sequence: exon 2, aa 28-91 (conformational epitope)
Entrez Gene ID: 6606 6607 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2618239 
Recommended Applications: ELISA, Immunofluorescence, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANSMA20(8C1) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANSMA20(8C1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    Epitope Map References
    All References

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