MO-1
Catalog Fields
Product Name/ID: MO-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531878
Clonality: Monoclonal
Immunogen: Adult rat ventral spinal cord homogenate
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Motor neuron marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/27/1998
Antigen Molecular Weight:
Depositor: Chiu, A.Y.
Antigen Sequence:
Depositor Institution: City of Hope, Beckman Research Institute
Antigen Species: Rat
Depositor Notes: This antibody recognizes a cytoplasmic antigen that is expressed in postnatal day 14 motor neurons in the brain stem and spinal cord. The antigen is expressed in neurons in functional contact with skeletal muscles.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information: RRID:AB_531878
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MO-1 was deposited to the DSHB by Chiu, A.Y. (DSHB Hybridoma Product MO-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
4 References
Initial Publication
A monoclonal antibody that recognizes somatic motor neurons in the mature rat nervous system.
Chiu AY
The Journal of neuroscience : the official journal of the Society for Neuroscience 10.2 (1990 Feb): 620-30.