MO-1

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$40.00
SKU: MO-1
View product citations for antibody MO-1 on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: Motor neuron marker
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Chiu, A.Y.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Beckman Research Institute of the City of Hope
Positive Tested Species Reactivity: Rat
Depositors Notes: This antibody recognizes a cytoplasmic antigen that is expressed in postnatal day 14 motor neurons in the brain stem and spinal cord. The antigen is expressed in neurons in functional contact with skeletal muscles.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Adult rat ventral spinal cord homogenate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531878 
Additional Information: RRID:AB_531878
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MO-1 was deposited to the DSHB by Chiu, A.Y. (DSHB Hybridoma Product MO-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    A monoclonal antibody that recognizes somatic motor neurons in the mature rat nervous system.
    Chiu AY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 10.2 (1990 Feb): 620-30.

    IF References

    A monoclonal antibody that recognizes somatic motor neurons in the mature rat nervous system.
    Chiu AY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 10.2 (1990 Feb): 620-30.

    A motor neuron-specific epitope and the low-affinity nerve growth factor receptor display reciprocal patterns of expression during development, axotomy, and regeneration.
    Loera S
    The Journal of comparative neurology 328.3 (1993 Feb 15): 351-63.

    Target regulation of a motor neuron-specific epitope.
    Chiu AY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.2 (1995 Feb): 1556-66.

    Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture.
    Hickman JJ
    Experimental neurology 209.1 (2008 Jan): 171-80.

    IHC References

    A monoclonal antibody that recognizes somatic motor neurons in the mature rat nervous system.
    Chiu AY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 10.2 (1990 Feb): 620-30.

    A motor neuron-specific epitope and the low-affinity nerve growth factor receptor display reciprocal patterns of expression during development, axotomy, and regeneration.
    Loera S
    The Journal of comparative neurology 328.3 (1993 Feb 15): 351-63.

    Target regulation of a motor neuron-specific epitope.
    Chiu AY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.2 (1995 Feb): 1556-66.

    All References

    A monoclonal antibody that recognizes somatic motor neurons in the mature rat nervous system.
    Chiu AY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 10.2 (1990 Feb): 620-30.

    A motor neuron-specific epitope and the low-affinity nerve growth factor receptor display reciprocal patterns of expression during development, axotomy, and regeneration.
    Loera S
    The Journal of comparative neurology 328.3 (1993 Feb 15): 351-63.

    Target regulation of a motor neuron-specific epitope.
    Chiu AY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.2 (1995 Feb): 1556-66.

    Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture.
    Hickman JJ
    Experimental neurology 209.1 (2008 Jan): 171-80.

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