Catalog Fields
Antigen: huntingtin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Patterson, P.H.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Human
Depositors Notes:
Predicted Species Reactivity:
Gene: HTT
Immunogen: Recombinant Hd exon-1 67Q/GST
Alternate Gene Names: HD, IT15
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: N-terminal, polyQ
Uniprot ID: P42858
Immunogen Sequence: Partial protein
Entrez Gene ID: 3064
Antibody Registry ID: AB_528295
Additional Information: On western blot, may recognize a breakdown product (250 to 300 kDa) of Htt.; Doesn't differentiate between HD and non-HD in Human.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MW6 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product MW6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins.
Patterson PH
Brain research bulletin 56.3-4 (2001 Oct-Nov 1): 319-29.