MW6

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$40.00
SKU: MW6
View product citations for antibody MW6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: huntingtin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Patterson, P.H.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Human
Depositors Notes:
Antigen Molecular Weight: Apparent: 348 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: HTT
Immunogen: Recombinant Hd exon-1 67Q/GST
Alternate Gene Names: HD, IT15
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: N-terminal, polyQ
Uniprot ID: P42858 
Immunogen Sequence: Partial protein
Entrez Gene ID: 3064 
Additional Characterization:  
Antibody Registry ID: AB_528295 
Additional Information: On western blot, may recognize a breakdown product (250 to 300 kDa) of Htt.; Doesn't differentiate between HD and non-HD in Human.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MW6 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product MW6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    Epitope Map References
    All References

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