MYO 231

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SKU: MYO 231

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DSHB Data Sheet

Catalog Fields

Product Name/ID: MYO 231
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_531854 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: wing bud ZPA (Zone of Polarizing Activity)
Clone:
Immunogen Sequence:
Myeloma Strain: P3X63 - Ag 8.653
Epitope Mapped: No
Antigen Name: muscle/neurite marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/17/1993
Antigen Molecular Weight:
Depositor: Carlson, B.M. / Carlson, J.A.
Antigen Sequence:
Depositor Institution: University of Michigan
Antigen Species: quail
Depositor Notes: 1. all quail myotomal myocytes at those stages (HH 16 through 24) and levels (trunk and hind-limb) tested; 2. neurites, but not cell bodies, of motor and sensory nerves, as well as commissural nerves of spinal cord; 3. limb muscles; and 4. occasionally in AER: ectodermal staining, but not robust. Does not stain: 1. neural crest; 2. notochord; 3. mesonephros; and 4. precartilagenous limb tissue.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Quail
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MYO 231 was deposited to the DSHB by Carlson, B.M. / Carlson, J.A. (DSHB Hybridoma Product MYO 231)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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