Antigen: muscle/neurite marker
Hybridoma Cells Available: Yes
Antigen Species: quail
Depositor: Carlson, B.M. / Carlson, J.A.
Host Species: mouse
Depositors Institution: University of Michigan
Positive Tested Species Reactivity: Quail
Depositors Notes: 1. all quail myotomal myocytes at those stages (HH 16 through 24) and levels (trunk and hind-limb) tested; 2. neurites, but not cell bodies, of motor and sensory nerves, as well as commissural nerves of spinal cord; 3. limb muscles; and 4. occasionally in AER: ectodermal staining, but not robust. Does not stain: 1. neural crest; 2. notochord; 3. mesonephros; and 4. precartilagenous limb tissue.
Predicted Species Reactivity:
Immunogen: wing bud ZPA (Zone of Polarizing Activity)
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63 - Ag 8.653
Epitope Location or Sequence:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MYO 231 was deposited to the DSHB by Carlson, B.M. / Carlson, J.A. (DSHB Hybridoma Product MYO 231)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.