Mab 44.1

(0) No Reviews yet
$45.00 to $425.00
SKU: Mab 44.1
View product citations for antibody Mab 44.1 on CiteAb

In Stock

Available: 12

DSHB Data Sheet

Catalog Fields

Antigen: Cytochrome b-245 light chain/ p22phox
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Jesaitis, A.J.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Montana State University
Positive Tested Species Reactivity: Human
Depositors Notes: The 44.1 antibody recognizes the native conformation of the p22phox subunit of Cytochrome b.
Antigen Molecular Weight: 22 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CYBA
Immunogen: Purified heterodimer Cytochrome b
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 3PU1
Epitope Location or Sequence: Conformational epitope: PQVRPI between 29-TAGRF-33 and 183-PQVNPI-188
Uniprot ID: P13498 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 1535 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: FACS, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 44.1 was deposited to the DSHB by Jesaitis, A.J. (DSHB Hybridoma Product Mab 44.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries.
    Jesaitis AJ
    The Journal of biological chemistry 270.28 (1995 Jul 14): 16974-80.

    IF References
    WB References

    Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries.
    Jesaitis AJ
    The Journal of biological chemistry 270.28 (1995 Jul 14): 16974-80.

    IP References
    FACS References

    Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries.
    Jesaitis AJ
    The Journal of biological chemistry 270.28 (1995 Jul 14): 16974-80.

    Epitope Map References

    Antibody imprint of a membrane protein surface. Phagocyte flavocytochrome b.
    Jesaitis AJ
    The Journal of biological chemistry 273.38 (1998 Sep 18): 24847-52.

    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review