Mab 54.1

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$40.00
SKU: Mab 54.1
View product citations for antibody Mab 54.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Cytochrome b-245 heavy chain/ gp91phox
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Jesaitis, A.J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Montana State University
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: The Mab 54.1 cross-reacts with Nox3 and GRP 58.
Antigen Molecular Weight: 91 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CYBB
Immunogen: Purified heterodimer Cytochrome b
Alternate Gene Names: NOX2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 3PU1
Epitope Location or Sequence: 383-PKIAVDGP-309
Uniprot ID: P04839 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 1536 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 54.1 was deposited to the DSHB by Jesaitis, A.J. (DSHB Hybridoma Product Mab 54.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • WB References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries.
    Jesaitis AJ
    The Journal of biological chemistry 270.28 (1995 Jul 14): 16974-80.

    WB References
    IP References

    Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries.
    Jesaitis AJ
    The Journal of biological chemistry 270.28 (1995 Jul 14): 16974-80.

    FACS References

    Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries.
    Jesaitis AJ
    The Journal of biological chemistry 270.28 (1995 Jul 14): 16974-80.

    Epitope Map References

    Antibody imprint of a membrane protein surface. Phagocyte flavocytochrome b.
    Jesaitis AJ
    The Journal of biological chemistry 273.38 (1998 Sep 18): 24847-52.

    All References

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