Mab NL7

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$45.00 to $425.00
SKU: Mab NL7
View product citations for antibody Mab NL7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Cytochrome b-245 heavy chain/ gp91phox
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Jesaitis, A.J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Montana State University
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes the cytoplasmic domain of the Cyt b large subunit gp91phox.
Antigen Molecular Weight: 91 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CYBB
Immunogen: Purified heterodimer Cytochrome b
Alternate Gene Names: NOX2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 3PU1
Epitope Location or Sequence: 498-EKDVITGLKQ-507
Uniprot ID: P04839 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 1536 
Additional Characterization:  
Antibody Registry ID:  
Additional Information: 16563510
Recommended Applications: FACS, Function Blocking, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab NL7 was deposited to the DSHB by Jesaitis, A.J. (DSHB Hybridoma Product Mab NL7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • WB References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Functional epitope on human neutrophil flavocytochrome b558.
    Jesaitis AJ
    Journal of immunology (Baltimore, Md. : 1950) 170.12 (2003 Jun 15): 6082-9.

    WB References

    Functional epitope on human neutrophil flavocytochrome b558.
    Jesaitis AJ
    Journal of immunology (Baltimore, Md. : 1950) 170.12 (2003 Jun 15): 6082-9.

    Monoclonal antibody 7D5 recognizes the R147 epitope on the gp91<sup>phox</sup> , phagocyte flavocytochrome b<sub>558</sub> large subunit.
    Kuribayashi F
    Microbiology and immunology 62.4 (2018 Apr): 269-280.

    Leishmania pifanoi amastigotes avoid macrophage production of superoxide by inducing heme degradation.
    Kima PE
    Infection and immunity 73.12 (2005 Dec): 8322-33.

    IP References
    FB References

    Functional epitope on human neutrophil flavocytochrome b558.
    Jesaitis AJ
    Journal of immunology (Baltimore, Md. : 1950) 170.12 (2003 Jun 15): 6082-9.

    Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit.
    Jesaitis AJ
    Journal of immunology (Baltimore, Md. : 1950) 173.12 (2004 Dec 15): 7349-57.

    Monoclonal antibody 7D5 recognizes the R147 epitope on the gp91<sup>phox</sup> , phagocyte flavocytochrome b<sub>558</sub> large subunit.
    Kuribayashi F
    Microbiology and immunology 62.4 (2018 Apr): 269-280.

    FACS References

    Functional epitope on human neutrophil flavocytochrome b558.
    Jesaitis AJ
    Journal of immunology (Baltimore, Md. : 1950) 170.12 (2003 Jun 15): 6082-9.

    NAD(P)H oxidase activity of Nox4 in chondrocytes is both inducible and involved in collagenase expression.
    Morel F
    Antioxidants & redox signaling 8.9-10 (2006 Sep-Oct): 1485-96.

    CRISPR/Cas9-mediated ASXL1 mutations in U937 cells disrupt myeloid differentiation.
    Young NS
    International journal of oncology 52.4 (2018 Apr): 1209-1223.

    Epitope Map References

    Functional epitope on human neutrophil flavocytochrome b558.
    Jesaitis AJ
    Journal of immunology (Baltimore, Md. : 1950) 170.12 (2003 Jun 15): 6082-9.

    All References

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