Mab PH1

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$40.00
SKU: Mab PH1
View product citations for antibody Mab PH1 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Verotoxin-1 B-subunit; Shiga-like toxin 1 B-subunit , Shiga toxin B-subunit
Hybridoma Cells Available: Yes
Antigen Species: Escherichia phage
Depositor: Lingwood, C. A. / Binnington, B.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Hospital for Sick Children, Toronto, CA
Positive Tested Species Reactivity: Any infected species
Depositors Notes: The beta subunit recognized by this antibody is a ligand for the globotriaosylceramide (Gb3, CD77) glycosphingolipid receptor.
Antigen Molecular Weight: pentamer 38.5 kDa; monomer (mature) 7.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: stxB
Immunogen: verotoxin 1 expressed by E. coli H30
Alternate Gene Names: sltB
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag 14
Epitope Location or Sequence:
Uniprot ID: P69179 
Immunogen Sequence: Full length protein
Entrez Gene ID: 26516283 
Additional Characterization: https://doi.org/10.3390/chromatography2030529 
Antibody Registry ID: AB_2713961 
Additional Information: Thin Layer Chromatography Overlay PMID: 7962116/11723119/10361298/8055965/ 18541659/10451385/ 9125554/20716521/ 26405105 RRID:AB_2713961
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab PH1 was deposited to the DSHB by Lingwood, C. A. / Binnington, B. (DSHB Hybridoma Product Mab PH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

23 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References

    Membrane cytosolic translocation of verotoxin A1 subunit in target cells.
    Lingwood CA
    Microbiology (Reading, England) 153.Pt 8 (2007 Aug): 2700-2710.

    ELISA References

    Adamantyl globotriaosyl ceramide: a monovalent soluble mimic which inhibits verotoxin binding to its glycolipid receptor.
    Lingwood CA
    Biochemical and biophysical research communications 257.2 (1999 Apr 13): 391-4.

    Interaction of the verotoxin 1B subunit with soluble aminodeoxy analogues of globotriaosyl ceramides.
    Lingwood CA
    The Biochemical journal 368.Pt 3 (2002 Dec 15): 769-76.

    Alpha-galactose based neoglycopeptides. Inhibition of verotoxin binding to globotriosylceramide.
    Peter MG
    Bioorganic & medicinal chemistry 7.12 (1999 Dec): 2823-33.

    Effect of globotriaosyl ceramide fatty acid alpha-hydroxylation on the binding by verotoxin 1 and verotoxin 2.
    Lingwood CA
    Neurochemical research 27.7-8 (2002 Aug): 807-13.

    Neutralization receptor-based immunoassay for detection of neutralizing antibodies to Escherichia coli verocytotoxin 1.
    Karmali M
    Journal of clinical microbiology 28.12 (1990 Dec): 2830-3.

    Oxidation of glycosphingolipids under basic conditions: synthesis of glycosyl "serine acids" as opposed to "ceramide acids". Precursors for neoglycoconjugates with increased ligand binding affinity.
    Lingwood CA
    Biochemistry 38.33 (1999 Aug 17): 10885-97.

    A comparison of the effects of verocytotoxin-1 on primary human renal cell cultures.
    Taylor CM
    Toxicology letters 105.1 (1999 Mar 8): 47-57.

    Lipid modulation of glycosphingolipid (GSL) receptors: soluble GSL mimics provide new probes of GSL receptor function.
    Mylvaganam M
    Methods in enzymology 363. (2003): 264-83.

    Localization of intravenously administered verocytotoxins (Shiga-like toxins) 1 and 2 in rabbits immunized with homologous and heterologous toxoids and toxin subunits.
    Petric M
    Infection and immunity 65.7 (1997 Jul): 2509-16.

    FB References
    All References

    Translocation of verotoxin-1 across T84 monolayers: mechanism of bacterial toxin penetration of epithelium.
    Sherman PM
    The American journal of physiology 273.6 (1997 Dec): G1349-58.

    Detergent-resistant globotriaosyl ceramide may define verotoxin/glomeruli-restricted hemolytic uremic syndrome pathology.
    Lingwood CA
    Kidney international 75.11 (2009 Jun): 1209-1216.

    The treatment of malignant meningioma with verotoxin.
    Van Furth WR
    Neoplasia (New York, N.Y.) 4.4 (2002 Jul-Aug): 304-11.

    Apparent cooperativity in multivalent verotoxin-globotriaosyl ceramide binding: kinetic and saturation binding studies with [(125)I]verotoxin.
    Lingwood CA
    Biochimica et biophysica acta 1501.2-3 (2000 Jun 15): 116-24.

    Verotoxin/globotriaosyl ceramide recognition: angiopathy, angiogenesis and antineoplasia.
    Lingwood CA
    Bioscience reports 19.5 (1999 Oct): 345-54.

    Brefeldin A and filipin distinguish two globotriaosyl ceramide/verotoxin-1 intracellular trafficking pathways involved in Vero cell cytotoxicity.
    Lingwood CA
    Glycobiology 14.8 (2004 Aug): 701-12.

    Verotoxin sensitivity of ECV304 cells in vitro and in vivo in a xenograft tumour model: VT1 as a tumour neovascular marker.
    Lingwood CA
    Angiogenesis 6.2 (2003): 129-41.

    Evidence that verotoxins (Shiga-like toxins) from Escherichia coli bind to P blood group antigens of human erythrocytes in vitro.
    Karmali MA
    Infection and immunity 62.8 (1994 Aug): 3337-47.

    A verotoxin 1 B subunit-lambda CRO chimeric protein specifically binds both DNA and globotriaosylceramide (Gb(3)) to effect nuclear targeting of exogenous DNA in Gb(3) positive cells.
    Lingwood CA
    Experimental cell research 269.1 (2001 Sep 10): 117-29.

    Experimental verocytotoxemia in rabbits.
    Karmali MA
    Infection and immunity 60.10 (1992 Oct): 4154-67.

    The identification of three biologically relevant globotriaosyl ceramide receptor binding sites on the Verotoxin 1 B subunit.
    Brunton JL
    Molecular microbiology 32.5 (1999 Jun): 953-60.

    Neutralization receptor-based immunoassay for detection of neutralizing antibodies to Escherichia coli verocytotoxin 1.
    Karmali M
    Journal of clinical microbiology 28.12 (1990 Dec): 2830-3.

    Phenylalanine 30 plays an important role in receptor binding of verotoxin-1.
    Brunton JL
    Molecular microbiology 19.4 (1996 Feb): 891-9.

    Membrane cytosolic translocation of verotoxin A1 subunit in target cells.
    Lingwood CA
    Microbiology (Reading, England) 153.Pt 8 (2007 Aug): 2700-2710.

    Adamantyl globotriaosyl ceramide: a monovalent soluble mimic which inhibits verotoxin binding to its glycolipid receptor.
    Lingwood CA
    Biochemical and biophysical research communications 257.2 (1999 Apr 13): 391-4.

    Interaction of the verotoxin 1B subunit with soluble aminodeoxy analogues of globotriaosyl ceramides.
    Lingwood CA
    The Biochemical journal 368.Pt 3 (2002 Dec 15): 769-76.

    Alpha-galactose based neoglycopeptides. Inhibition of verotoxin binding to globotriosylceramide.
    Peter MG
    Bioorganic & medicinal chemistry 7.12 (1999 Dec): 2823-33.

    Effect of globotriaosyl ceramide fatty acid alpha-hydroxylation on the binding by verotoxin 1 and verotoxin 2.
    Lingwood CA
    Neurochemical research 27.7-8 (2002 Aug): 807-13.

    Oxidation of glycosphingolipids under basic conditions: synthesis of glycosyl "serine acids" as opposed to "ceramide acids". Precursors for neoglycoconjugates with increased ligand binding affinity.
    Lingwood CA
    Biochemistry 38.33 (1999 Aug 17): 10885-97.

    A comparison of the effects of verocytotoxin-1 on primary human renal cell cultures.
    Taylor CM
    Toxicology letters 105.1 (1999 Mar 8): 47-57.

    Lipid modulation of glycosphingolipid (GSL) receptors: soluble GSL mimics provide new probes of GSL receptor function.
    Mylvaganam M
    Methods in enzymology 363. (2003): 264-83.

    Localization of intravenously administered verocytotoxins (Shiga-like toxins) 1 and 2 in rabbits immunized with homologous and heterologous toxoids and toxin subunits.
    Petric M
    Infection and immunity 65.7 (1997 Jul): 2509-16.

    Differential tissue targeting and pathogenesis of verotoxins 1 and 2 in the mouse animal model.
    Lingwood CA
    Kidney international 62.3 (2002 Sep): 832-45.

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