Mab PH1
Catalog Fields
Clone ID/Product Name: Mab PH1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: stxB
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_2713961
Uniprot ID: P69179
Entrez Gene ID: 26516283
Clonality: Monoclonal
Immunogen: verotoxin 1 expressed by E. coli H30
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0-Ag 14
Epitope Mapped: No
Antigen Name: Verotoxin-1 B-subunit; Shiga-like toxin 1 B-subunit , Shiga toxin B-subunit
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/13/2018
Antigen Molecular Weight: pentamer 38.5 kDa; monomer (mature) 7.7 kDa
Depositor: Lingwood, C. A. / Binnington, B.
Antigen Sequence:
Depositor Institution: Hospital for Sick Children
Antigen Species: Escherichia phage
Depositor Notes: The beta subunit recognized by this antibody is a ligand for the globotriaosylceramide (Gb3, CD77) glycosphingolipid receptor.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Any infected species
Additional Information: Thin Layer Chromatography Overlay PMID: 7962116/11723119/10361298/8055965/ 18541659/10451385/ 9125554/20716521/ 26405105 RRID:AB_2713961
Additional Characterization: https://doi.org/10.3390/chromatography2030529
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab PH1 was deposited to the DSHB by Lingwood, C. A. / Binnington, B. (DSHB Hybridoma Product Mab PH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
23 References
Initial Publication
Neutralization receptor-based immunoassay for detection of neutralizing antibodies to Escherichia coli verocytotoxin 1.
Karmali M
Journal of clinical microbiology 28.12 (1990 Dec): 2830-3.