N1.551

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SKU: N1.551

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: N1.551
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: MYH2
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_2147780 
Uniprot ID: Q9UKX2 
RRID:  
Entrez Gene ID: 4620 
Clonality: Monoclonal
Immunogen: partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/2
Epitope Mapped: No
Antigen Name: Myosin heavy chain Type IIA fast and human neonatal
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/25/1993
Antigen Molecular Weight: 220kDa
Depositor: Blau, H.M.
Antigen Sequence:
Depositor Institution: Stanford University, Baxter Lab for Stem Cell Biology
Antigen Species: Human
Depositor Notes: Fusion: 1983. Recognizes IIA fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX fibers, and doesn't stain IIB.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Human, Rodent, Zebrafish
Additional Information: This antibody has been reported to also bind neonatal MyHC. It may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N1.551 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product N1.551)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions.
    Mantilla CB
    Respiratory physiology & neurobiology 180.1 (2012 Jan 15): 88-96.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Distinct mechanisms regulate slow-muscle development.
    Devoto SH
    Current biology : CB 11.18 (2001 Sep 18): 1432-8.

    Molecular analysis of fiber type-specific expression of murine myostatin promoter.
    Sharma M
    American journal of physiology. Cell physiology 287.4 (2004 Oct): C1031-40.

    Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle.
    Kaminski HJ
    The Journal of physiology 589.Pt 18 (2011 Sep 15): 4601-14.

    Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.
    Kaminski HJ
    Investigative ophthalmology & visual science 51.12 (2010 Dec): 6355-63.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception.
    Blau HM
    Journal of cell science 107 ( Pt 9). (1994 Sep): 2361-71.

    WB References
    IHC References

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle & nerve 49.4 (2014 Apr): 534-44.

    BDNF rescues myosin heavy chain IIB muscle fibers after neonatal nerve injury.
    Jasmin BJ
    American journal of physiology. Cell physiology 287.1 (2004 Jul): C22-9.

    ELISA References
    All References

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle & nerve 49.4 (2014 Apr): 534-44.

    BDNF rescues myosin heavy chain IIB muscle fibers after neonatal nerve injury.
    Jasmin BJ
    American journal of physiology. Cell physiology 287.1 (2004 Jul): C22-9.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions.
    Mantilla CB
    Respiratory physiology & neurobiology 180.1 (2012 Jan 15): 88-96.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Distinct mechanisms regulate slow-muscle development.
    Devoto SH
    Current biology : CB 11.18 (2001 Sep 18): 1432-8.

    Molecular analysis of fiber type-specific expression of murine myostatin promoter.
    Sharma M
    American journal of physiology. Cell physiology 287.4 (2004 Oct): C1031-40.

    Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle.
    Kaminski HJ
    The Journal of physiology 589.Pt 18 (2011 Sep 15): 4601-14.

    Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.
    Kaminski HJ
    Investigative ophthalmology & visual science 51.12 (2010 Dec): 6355-63.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception.
    Blau HM
    Journal of cell science 107 ( Pt 9). (1994 Sep): 2361-71.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

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