N1.551

(0) No Reviews yet
$40.00
SKU: N1.551
View product citations for antibody N1.551 on CiteAb

In Stock

Available: 24

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (human neonatal fast IIa fibers)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baxter Lab for Stem Cell Biology, Stanford University
Positive Tested Species Reactivity: Bovine, Human, Rodent, Zebrafish
Depositors Notes: Fusion: 1983. Recognizes IIA fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX fibers, and doesn't stain IIB.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle
Alternate Gene Names: BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/2
Epitope Location or Sequence:
Uniprot ID: Q9UKX2 
Immunogen Sequence:
Entrez Gene ID: 4620 
Additional Characterization:
Antibody Registry ID: AB_2147780 
Additional Information: This antibody has been reported to also bind neonatal MyHC. It may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N1.551 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product N1.551)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions.
    Mantilla CB
    Respiratory physiology and neurobiology 180.1 (2012 Jan 15): 88-96.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Distinct mechanisms regulate slow-muscle development.
    Devoto SH
    Current biology : CB 11.18 (2001 Sep 18): 1432-8.

    Molecular analysis of fiber type-specific expression of murine myostatin promoter.
    Sharma M
    American journal of physiology. Cell physiology 287.4 (2004 Oct): C1031-40.

    Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle.
    Kaminski HJ
    The Journal of physiology 589.Pt 18 (2011 Sep 15): 4601-14.

    Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.
    Kaminski HJ
    Investigative ophthalmology and visual science 51.12 (2010 Dec): 6355-63.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    WB References
    IHC References

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle and nerve 49.4 (2014 Apr): 534-44.

    BDNF rescues myosin heavy chain IIB muscle fibers after neonatal nerve injury.
    Jasmin BJ
    American journal of physiology. Cell physiology 287.1 (2004 Jul): C22-9.

    ELISA References
    All References

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle and nerve 49.4 (2014 Apr): 534-44.

    BDNF rescues myosin heavy chain IIB muscle fibers after neonatal nerve injury.
    Jasmin BJ
    American journal of physiology. Cell physiology 287.1 (2004 Jul): C22-9.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions.
    Mantilla CB
    Respiratory physiology and neurobiology 180.1 (2012 Jan 15): 88-96.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Distinct mechanisms regulate slow-muscle development.
    Devoto SH
    Current biology : CB 11.18 (2001 Sep 18): 1432-8.

    Molecular analysis of fiber type-specific expression of murine myostatin promoter.
    Sharma M
    American journal of physiology. Cell physiology 287.4 (2004 Oct): C1031-40.

    Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle.
    Kaminski HJ
    The Journal of physiology 589.Pt 18 (2011 Sep 15): 4601-14.

    Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.
    Kaminski HJ
    Investigative ophthalmology and visual science 51.12 (2010 Dec): 6355-63.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review