Product Name/ID: N168/6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SCN4B
Ab Isotype: MIgG1
Uniprot ID: Q7M730
Entrez Gene ID: 315611
Immunogen: Fusion protein
Immunogen Sequence: a.a. 184-226
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Navbeta4 Na+ channel
Epitope Location or Sequence: Cytoplasmic C-terminus
Alternate Antigen Name:
Deposit Date: 11/10/2021
Antigen Molecular Weight: 25.2 kDa
Depositor: Trimmer, J.S.
Depositor Institution: UC - Davis
Antigen Species: Rat
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse, Rat
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N168_6.pdf
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N168/6 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N168/6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.