N23B/49

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$45.00
SKU: N23B/49
View product citations for antibody N23B/49 on CiteAb

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Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: Pan-Shank
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Recognizes Shank1, Shank2 and Shank3
Antigen Molecular Weight: 160 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Shank2
Immunogen: Fusion Protein
Alternate Gene Names: CortBP1, ProSAP1, Spank-3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence: SH3/PDZ domains)
Uniprot ID: Q9QX74 
Immunogen Sequence: a.a. 84-308
Entrez Gene ID: 171093 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N23B/49 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N23B/49)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Requirement for protein synthesis at developing synapses.
    Benson DL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.31 (2009 Aug 5): 9778-93.

    All References

    Requirement for protein synthesis at developing synapses.
    Benson DL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.31 (2009 Aug 5): 9778-93.

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