Antigen: P granule abnormality protein 1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Strome, S.
Host Species: mouse
Depositors Institution: University of California Santa Cruz
Positive Tested Species Reactivity: C. elegans, Planaria
Depositors Notes: Antibody binds to P granules in specimens fixed with either methanol/acetone or aldehydes.
Antigen Molecular Weight: 40 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: C. elegans embryo homogenate
Alternate Gene Names: ZK381.4
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: P3X63Ag8
Epitope Location or Sequence:
Uniprot ID: Q9TZQ3
Immunogen Sequence: Full length protein
Entrez Gene ID: 177461
Antibody Registry ID: AB_531837
Additional Information: RRID:AB_531837
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
OIC1D4 was deposited to the DSHB by Strome, S. (DSHB Hybridoma Product OIC1D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).