OIC1D4

(0)No Reviews yet
$50.00 to $600.00
SKU: OIC1D4

In Stock

Available: 180

DSHB Data Sheet

Catalog Fields

Product Name/ID: OIC1D4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: pgl-1
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_531837 
Uniprot ID: Q9TZQ3 
RRID:  
Entrez Gene ID: 177461 
Clonality: Monoclonal
Immunogen: C. elegans embryo homogenate
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8
Epitope Mapped: No
Antigen Name: P granule abnormality protein 1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/18/1994
Antigen Molecular Weight: 40 kDa
Depositor: Strome, S.
Antigen Sequence:
Depositor Institution: University of California, Santa Cruz
Antigen Species: C. elegans
Depositor Notes: Antibody binds to P granules in specimens fixed with either methanol/acetone or aldehydes.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans, Planaria
Additional Information: RRID:AB_531837
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
OIC1D4 was deposited to the DSHB by Strome, S. (DSHB Hybridoma Product OIC1D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
    Strome S
    Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.

    IF References

    Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
    Strome S
    Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.

    Characterization of a germ-line proliferation mutation in C. elegans.
    Strome S
    Development (Cambridge, England) 116.3 (1992 Nov): 755-66.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development.
    Evans TC
    The Journal of cell biology 182.3 (2008 Aug 11): 559-72.

    Linker histone HIS-24 (H1.1) cytoplasmic retention promotes germ line development and influences histone H3 methylation in Caenorhabditis elegans.
    Schulze E
    Molecular and cellular biology 27.6 (2007 Mar): 2229-39.

    The Sm proteins regulate germ cell specification during early C. elegans embryogenesis.
    Evans TC
    Developmental biology 291.1 (2006 Mar 1): 132-43.

    H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans.
    Salcini AE
    Nucleic acids research 43.20 (2015 Nov 16): 9694-710.

    LSY-2 is essential for maintaining the germ-soma distinction in C. elegans.
    Zhang H
    Protein & cell 6.8 (2015 Aug): 599-609.

    C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization.
    Chartier NT
    The Journal of cell biology 206.1 (2014 Jul 7): 129-43.

    The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans.
    Reinke V
    Genetics 208.2 (2018 Feb): 549-563.

    All References

    Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
    Strome S
    Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.

    Characterization of a germ-line proliferation mutation in C. elegans.
    Strome S
    Development (Cambridge, England) 116.3 (1992 Nov): 755-66.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development.
    Evans TC
    The Journal of cell biology 182.3 (2008 Aug 11): 559-72.

    Linker histone HIS-24 (H1.1) cytoplasmic retention promotes germ line development and influences histone H3 methylation in Caenorhabditis elegans.
    Schulze E
    Molecular and cellular biology 27.6 (2007 Mar): 2229-39.

    The Sm proteins regulate germ cell specification during early C. elegans embryogenesis.
    Evans TC
    Developmental biology 291.1 (2006 Mar 1): 132-43.

    H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans.
    Salcini AE
    Nucleic acids research 43.20 (2015 Nov 16): 9694-710.

    LSY-2 is essential for maintaining the germ-soma distinction in C. elegans.
    Zhang H
    Protein & cell 6.8 (2015 Aug): 599-609.

    C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization.
    Chartier NT
    The Journal of cell biology 206.1 (2014 Jul 7): 129-43.

    The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans.
    Reinke V
    Genetics 208.2 (2018 Feb): 549-563.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review