Catalog Fields
Antigen: P granule abnormality protein 1
Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: Strome, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. elegans, Planaria
Depositors Institution: University of California, Santa Cruz
Antigen Molecular Weight: 40 kDa
Depositors Notes: Antibody binds to P granules in specimens fixed with either methanol/acetone or aldehydes.
Immunogen: C. elegans embryo homogenate
Gene: pgl-1
Alternate Antibody Name:
Alternate Gene Names: ZK381.4
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8
Epitope Mapped: No
Uniprot ID: Q9TZQ3
Epitope Location or Sequence:
Entrez Gene ID: 177461
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_531837
Recommended Applications: Immunofluorescence
Additional Information: RRID:AB_531837
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
OIC1D4 was deposited to the DSHB by Strome, S. (DSHB Hybridoma Product OIC1D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
Strome S
Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.