OIC1D4

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$40.00
SKU: OIC1D4
View product citations for antibody OIC1D4 on CiteAb

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Available: 154

DSHB Data Sheet

Catalog Fields

Antigen: P granule abnormality protein 1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Strome, S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California Santa Cruz
Positive Tested Species Reactivity: C. elegans, Planaria
Depositors Notes: Antibody binds to P granules in specimens fixed with either methanol/acetone or aldehydes.
Antigen Molecular Weight: 40 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: pgl-1
Immunogen: C. elegans embryo homogenate
Alternate Gene Names: ZK381.4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8
Epitope Location or Sequence:
Uniprot ID: Q9TZQ3 
Immunogen Sequence: Full length protein
Entrez Gene ID: 177461 
Additional Characterization:  
Antibody Registry ID: AB_531837 
Additional Information: RRID:AB_531837
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
OIC1D4 was deposited to the DSHB by Strome, S. (DSHB Hybridoma Product OIC1D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
    Strome S
    Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.

    IF References

    Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
    Strome S
    Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.

    Characterization of a germ-line proliferation mutation in C. elegans.
    Strome S
    Development (Cambridge, England) 116.3 (1992 Nov): 755-66.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development.
    Evans TC
    The Journal of cell biology 182.3 (2008 Aug 11): 559-72.

    Linker histone HIS-24 (H1.1) cytoplasmic retention promotes germ line development and influences histone H3 methylation in Caenorhabditis elegans.
    Schulze E
    Molecular and cellular biology 27.6 (2007 Mar): 2229-39.

    The Sm proteins regulate germ cell specification during early C. elegans embryogenesis.
    Evans TC
    Developmental biology 291.1 (2006 Mar 1): 132-43.

    H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans.
    Salcini AE
    Nucleic acids research 43.20 (2015 Nov 16): 9694-710.

    LSY-2 is essential for maintaining the germ-soma distinction in C. elegans.
    Zhang H
    Protein & cell 6.8 (2015 Aug): 599-609.

    C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization.
    Chartier NT
    The Journal of cell biology 206.1 (2014 Jul 7): 129-43.

    The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in <i>Caenorhabditis elegans</i>.
    Reinke V
    Genetics 208.2 (2018 Feb): 549-563.

    All References

    Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
    Strome S
    Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.

    Characterization of a germ-line proliferation mutation in C. elegans.
    Strome S
    Development (Cambridge, England) 116.3 (1992 Nov): 755-66.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development.
    Evans TC
    The Journal of cell biology 182.3 (2008 Aug 11): 559-72.

    Linker histone HIS-24 (H1.1) cytoplasmic retention promotes germ line development and influences histone H3 methylation in Caenorhabditis elegans.
    Schulze E
    Molecular and cellular biology 27.6 (2007 Mar): 2229-39.

    The Sm proteins regulate germ cell specification during early C. elegans embryogenesis.
    Evans TC
    Developmental biology 291.1 (2006 Mar 1): 132-43.

    H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans.
    Salcini AE
    Nucleic acids research 43.20 (2015 Nov 16): 9694-710.

    LSY-2 is essential for maintaining the germ-soma distinction in C. elegans.
    Zhang H
    Protein & cell 6.8 (2015 Aug): 599-609.

    C. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization.
    Chartier NT
    The Journal of cell biology 206.1 (2014 Jul 7): 129-43.

    The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in <i>Caenorhabditis elegans</i>.
    Reinke V
    Genetics 208.2 (2018 Feb): 549-563.

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