OIC1D4
Catalog Fields
Antigen: P granule abnormality protein 1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Strome, S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California Santa Cruz
Positive Tested Species Reactivity: C. elegans, Planaria
Depositors Notes: Antibody binds to P granules in specimens fixed with either methanol/acetone or aldehydes.
Predicted Species Reactivity:
Gene: pgl-1
Immunogen: C. elegans embryo homogenate
Alternate Gene Names: ZK381.4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8
Epitope Location or Sequence:
Uniprot ID: Q9TZQ3
Immunogen Sequence: Full length protein
Entrez Gene ID: 177461
Antibody Registry ID: AB_531837
Additional Information: RRID:AB_531837
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
OIC1D4 was deposited to the DSHB by Strome, S. (DSHB Hybridoma Product OIC1D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
10 References
Initial Publication
Asymmetric movements of cytoplasmic components in Caenorhabditis elegans zygotes.
Strome S
Journal of embryology and experimental morphology 97 Suppl. (1986 Oct): 15-29.