ORC2

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$40.00
SKU: ORC2
View product citations for antibody ORC2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ORC-2
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works on C. elegans whole mounts. Stains nuclei.
Antigen Molecular Weight: Predicted: 49 kDa; Apparent: 49 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: orc-2
Immunogen: Recombinant His6-tagged ORC-2 fusion protein
Alternate Gene Names:
Alternate Antibody Name: 4D4
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q21037 
Immunogen Sequence: full length protein
Entrez Gene ID: 174676 
Additional Characterization:  
Antibody Registry ID: AB_10567263 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ORC2 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product ORC2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

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