Available to For-Profits: Yes
Antigen Species: rat
Hybridoma Cells Available (Non-Profit): No
Depositor: McConnell, S.K.
Host Species: mouse
Positive Tested Species Reactivity: Ferret, Mouse, Rat
Depositors Institution: Stanford University
Antigen Molecular Weight: 40kDa
Depositors Notes: Antigen note: homeodomain transcription factor. IHC note: subfraction of neurons in layers 5 and 6 in adult rodent cortex, developmentally regulated. Functional effects: axon remodeling?
Immunogen: rat Otx1, C terminus residues 118-269
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Myeloma Strain: NS-1
Epitope Mapped: No
Antibody Registry ID: AB_528423
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Otx-5F5 was deposited to the DSHB by McConnell, S.K. (DSHB Hybridoma Product Otx-5F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).