P1H11

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SKU: P1H11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: P1H11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: FN1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619588 
Uniprot ID: P02751 
RRID:  
Entrez Gene ID: 2335 
Clonality: Monoclonal
Immunogen: Purified human fibronectin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Antigen Name: fibronectin, cell binding domain
Epitope Location or Sequence: Cell binding domain (aa 1267-1540 of isoform 1) [see Depositor Notes]
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Predicted: multiple isoforms between 218 and 256 kDa and one or two isoforms at 69-71 kDa, plus shorter cleavage products.
Depositor: Wayner, E.A.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: Frozen (acetone), paraformaldehyde, and methanol fixation work well for immunostaining. Doesn't appear to work for ELISA nor Flow cytometry. The MW observed by western blotting was 220 kDa (38 kDa and 190 kDa fibronectin A chain precursor). The epitope was mapped to the cell binding domain.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Primate, Rat
Additional Information: Acetone or paraformaldehyde fixation
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1H11 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P1H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References

    Basal prostate epithelial cells stimulate the migration of prostate cancer cells.
    Knudsen BS
    Molecular carcinogenesis 41.2 (2004 Oct): 85-97.

    All References

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