Antibody Name: P1H11
Antigen:fibronectin, cell binding domain
Catalog Fields
Identity
Clone ID / Antibody Name: P1H11
Alternate Antibody Name: —
Antigen Name: fibronectin, cell binding domain
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: FN1
Entrez Gene ID: 2335
Antigen Sequence: —
Antigen Species: Human
Antigen Mol. Weight: Predicted: multiple isoforms between 218 and 256 kDa and one or two isoforms at 69-71 kDa, plus shorter cleavage products.
Uniprot ID: P02751
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: NS-1/FOX-NY
Antibody Registry / RRID: AB_2619588
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Purified human fibronectin
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: Cell binding domain (aa 1267-1540 of isoform 1) [see Depositor Notes]
Clone: —
Additional Info: Acetone or paraformaldehyde fixation
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Human, Mouse, Primate, Rat
Predicted Reactivity: —
Depositor
Depositor Name: E.A. Wayner
Institution: Fred Hutchinson Cancer Research Center
Deposit Date: October 11, 2011
Depositor Notes: Frozen (acetone), paraformaldehyde, and methanol fixation work well for immunostaining. Doesn't appear to work for ELISA nor Flow cytometry. The MW observed by western blotting was 220 kDa (38 kDa and 190 kDa fibronectin A chain precursor). The epitope was mapped to the cell binding domain.
Recommended Applications
FFPE
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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