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SKU: P4G11
View product citations for antibody P4G11 on CiteAb

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Available: 192

DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 (CD29), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1
Depositors Institution: Fred Hutchison Cancer Research Center
Host Species: mouse
Depositors Notes: Stimulates binding of ?1 integrins. This antibody doesn't appear to work for western blotting.
Positive Tested Species Reactivity: Human
Gene: ITGB1
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Alternate Gene Names: CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA
Immunogen: Human monocytic U937 cells
Clonality: Monoclonal
Alternate Antibody Name: CD29
Epitope Mapped: Yes
Myeloma Strain: Fox NY
Epitope Location or Sequence: extracellular
Uniprot ID: P05556 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3688 
Additional Information: P4G11 is not a function blocking mAb. It's functional effect is enhancing. P4G11 recognizes a Ca2+-dependent epitope.
Antibody Registry ID: AB_528307 
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4G11 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P4G11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    FB References
    FACS References
    Epitope Map References
    All References

    Chlamydia trachomatis does not bind to alpha beta 1 integrins to colonize a human endometrial epithelial cell line cultured in vitro.
    Wayner EA
    Microbial pathogenesis 17.3 (1994 Sep): 159-66.

    Binding of αvβ1 and αvβ6 integrins to tenascin-C induces epithelial-mesenchymal transition-like change of breast cancer cells.
    Yoshida T
    Oncogenesis 2. (2013 Aug 19): e65.

    Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
    Carter WG
    The Journal of cell biology 121.5 (1993 Jun): 1141-52.

    SNARE-dependent interaction of Src, EGFR and β1 integrin regulates invadopodia formation and tumor cell invasion.
    Coppolino MG
    Journal of cell science 127.Pt 8 (2014 Apr 15): 1712-25.

    Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1).
    LeBien TW
    Blood 81.9 (1993 May 1): 2272-82.

    3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments.
    Werner C
    Acta biomaterialia 36. (2016 May): 73-85.

    Cross-talk of integrin alpha3beta1 and tissue factor in cell migration.
    Ruf W
    Molecular biology of the cell 15.10 (2004 Oct): 4416-25.

    Early infection HIV-1 envelope V1-V2 genotypes do not enhance binding or replication in cells expressing high levels of α4β7 integrin.
    Sagar M
    Journal of acquired immune deficiency syndromes (1999) 64.3 (2013 Nov 1): 249-53.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

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