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SKU: P4G11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: P4G11
Available to For-Profits: Yes
Alternate Antibody Name: CD29
Gene Name: ITGB1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528307 
Uniprot ID: P05556 
Entrez Gene ID: 3688 
Clonality: Monoclonal
Immunogen: Human monocytic U937 cells
Immunogen Sequence: Full length protein
Myeloma Strain: Fox NY
Epitope Mapped: Yes
Antigen Name: integrin beta-1 (CD29), human
Epitope Location or Sequence: extracellular
Alternate Antigen Name:
Deposit Date: 8/4/1993
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Depositor: Wayner, E.A.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: Stimulates binding of ?1 integrins. This antibody doesn't appear to work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: P4G11 is not a function blocking mAb. It's functional effect is enhancing. P4G11 recognizes a Ca2+-dependent epitope.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4G11 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P4G11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    FB References
    FACS References
    Epitope Map References
    All References

    Chlamydia trachomatis does not bind to alpha beta 1 integrins to colonize a human endometrial epithelial cell line cultured in vitro.
    Wayner EA
    Microbial pathogenesis 17.3 (1994 Sep): 159-66.

    Binding of αvβ1 and αvβ6 integrins to tenascin-C induces epithelial-mesenchymal transition-like change of breast cancer cells.
    Yoshida T
    Oncogenesis 2.8 (2013 Aug 19): e65.

    Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
    Carter WG
    The Journal of cell biology 121.5 (1993 Jun): 1141-52.

    SNARE-dependent interaction of Src, EGFR and β1 integrin regulates invadopodia formation and tumor cell invasion.
    Coppolino MG
    Journal of cell science 127.Pt 8 (2014 Apr 15): 1712-25.

    Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1).
    LeBien TW
    Blood 81.9 (1993 May 1): 2272-82.

    3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments.
    Werner C
    Acta biomaterialia 36. (2016 May): 73-85.

    Cross-talk of integrin alpha3beta1 and tissue factor in cell migration.
    Ruf W
    Molecular biology of the cell 15.10 (2004 Oct): 4416-25.

    Early infection HIV-1 envelope V1-V2 genotypes do not enhance binding or replication in cells expressing high levels of α4β7 integrin.
    Sagar M
    Journal of acquired immune deficiency syndromes (1999) 64.3 (2013 Nov 1): 249-53.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

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