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View product citations for antibody P4H9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG3
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Human
Depositors Notes: Inhibits leukocyte binding to ICAM. Immunostaining: methanol fixation. Binds hematopoietic cells.
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGB2
Immunogen: human lymphokine activated T-cells (LAK)
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Alternate Antibody Name: 60.3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: BALB/c MOPC2 NSI/1
Epitope Location or Sequence:
Uniprot ID: P05107 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3689 
Additional Characterization:  
Antibody Registry ID: AB_2619602 
Additional Information: CD18 is a general marker of leukocytes.
Recommended Applications: FACS, Function Blocking, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4H9 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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