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View product citations for antibody P4H9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG3
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Human
Depositors Notes: Inhibits leukocyte binding to ICAM. Immunostaining: methanol fixation. Binds hematopoietic cells.
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ITGB2
Immunogen: human lymphokine activated T-cells (LAK)
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Alternate Antibody Name: 60.3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: BALB/c MOPC2 NSI/1
Epitope Location or Sequence:
Uniprot ID: P05107 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3689 
Additional Characterization:
Antibody Registry ID: AB_2619602 
Additional Information: CD18 is a general marker of leukocytes.
Recommended Applications: FACS, Function Blocking, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4H9 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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