P4H9

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$50.00
SKU: P4H9

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: P4H9
Available to For-Profits: Yes
Alternate Antibody Name: 60.3
Gene Symbol: ITGB2
Ab Isotype: MIgG3
Gene Name:
Antibody Registry ID: AB_2619602 
Uniprot ID: P05107 
RRID:  
Entrez Gene ID: 3689 
Clonality: Monoclonal
Immunogen: human lymphokine activated T-cells (LAK)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: BALB/c MOPC2 NSI/1
Epitope Mapped: No
Antigen Name: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Depositor: Wayner, E.A. / Carter, W.G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: Inhibits leukocyte binding to ICAM. Immunostaining: methanol fixation. Binds hematopoietic cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: CD18 is a general marker of leukocytes.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4H9 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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