PCRP-BRWD1-1E12

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$40.00
SKU: PCRP-BRWD1-1E12
View product citations for antibody PCRP-BRWD1-1E12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Bromodomain and WD repeat domain containing 1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2a
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: This antibody recognizes a member of theWD repeat protein family.
Positive Tested Species Reactivity: Human
Gene: BRWD1
Antigen Molecular Weight: 262.9 kDa
Alternate Gene Names: N143, WDR9
Immunogen: Recombinant peptide
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Uniprot ID: Q9NSI6 
Immunogen Sequence: a.a. 1293-1450
Entrez Gene ID: 54014 
Additional Information: RRID:AB_2618456
Antibody Registry ID: AB_2618456 
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-BRWD1-1E12 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-BRWD1-1E12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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