PCRP-CBX3-2E1

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$40.00
SKU: PCRP-CBX3-2E1
View product citations for antibody PCRP-CBX3-2E1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Chromobox homolog 3
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG1
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: This antigen is also known as Rectachrome 1 or Polycomb 3 homolog.
Positive Tested Species Reactivity: Human
Gene: CBX3
Antigen Molecular Weight: 20.81 kDa
Alternate Gene Names: HP1Hs-gamma
Predicted Species Reactivity: Human, Mouse
Clonality: Monoclonal
Immunogen: Recombinant protein
Immunogen Sequence: Full length
Myeloma Strain: SP2/0-Ag14
Additional Information: RRID: AB_2618475
Uniprot ID: Q13185 
Entrez Gene ID: 11335 
Antibody Registry ID: AB_2618475 
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-CBX3-2E1 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-CBX3-2E1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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