PCRP-CC2D1A-1C2

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SKU: PCRP-CC2D1A-1C2

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DSHB Data Sheet

Catalog Fields

Product Name/ID: PCRP-CC2D1A-1C2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CC2D1A
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2618480 
Uniprot ID: Q6P1N0 
RRID:  
Entrez Gene ID: 54862 
Clonality: Monoclonal
Immunogen: Recombinant protein
Clone:
Immunogen Sequence: Full length
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: Coiled-coil and C2 domain containing 1A
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/1/2015
Antigen Molecular Weight: 104.06
Depositor: Common Fund – Protein Capture Reagents Program
Antigen Sequence:
Depositor Institution: National Institutes of Health
Antigen Species: Human
Depositor Notes: This antigen is a transcription factor that binds specificically with the dual repressor element in neuronal cells. In the cytoplasm, this protein acts as a signaling scaffold protein.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_2618480
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Microarray, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-CC2D1A-1C2 was deposited to the DSHB by Common Fund – Protein Capture Reagents Program (DSHB Hybridoma Product PCRP-CC2D1A-1C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

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