PCRP-CIAO1-2C11

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$40.00
SKU: PCRP-CIAO1-2C11
View product citations for antibody PCRP-CIAO1-2C11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Cytosolic iron-sulfur assembly component 1
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG1
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: This antigen is also know as probable cytosolic iron-sulofur protein assembly protein CIAO1.
Positive Tested Species Reactivity: Human
Gene: CIAO1
Antigen Molecular Weight: 37.84 kDa
Alternate Gene Names: CIA1, WDR39
Immunogen: Recombinant protein
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Uniprot ID: O76071 
Immunogen Sequence: Full length
Entrez Gene ID: 9391 
Additional Information: This antibody has been characterized by the NIH Protein Capture Reagents Program. RRID:AB_2722145
Antibody Registry ID: AB_2722145 
Recommended Applications: ELISA, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-CIAO1-2C11 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-CIAO1-2C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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