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View product citations for antibody PCRP-FEZF2-1B1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: FEZ family zinc finger 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG1
Depositors Institution: Protein Capture Reagents Program, produced by JHU/CDI
Host Species: mouse
Gene: FEZF2
Positive Tested Species Reactivity: Human
Alternate Gene Names: FEZL, TOF, Zfp312, ZNF312
Antigen Molecular Weight: 48.81 kDa
Clonality: Monoclonal
Predicted Species Reactivity: Human, Mouse, Rat
Epitope Mapped: No
Immunogen: Recombinant protein
Immunogen Sequence: Protein Domain
Myeloma Strain: SP2/0-Ag14
Additional Information: RRID: AB_2618617
Uniprot ID: Q8TBJ5 
Entrez Gene ID: 55079 
Antibody Registry ID: AB_2618617 
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-FEZF2-1B1 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-FEZF2-1B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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