(0) No Reviews yet
View product citations for antibody PCRP-NFATC3-2A12 on CiteAb

In Stock

Available: 71

DSHB Data Sheet

Catalog Fields

Antigen: Nuclear factor of activated T-cells, cytoplasmic 3
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2b
Depositors Institution: National Institutes of Health
Host Species: mouse
Depositors Notes: The antigen is also known as Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3.
Positive Tested Species Reactivity: Human
Gene: NFATC3
Antigen Molecular Weight: 115.59 kDa
Alternate Gene Names: NFAT4, NFATX
Predicted Species Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Additional Information: RRID: AB_2618881
Uniprot ID: Q12968 
Entrez Gene ID: 4775 
Antibody Registry ID: AB_2618881 
Recommended Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-NFATC3-2A12 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-NFATC3-2A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review