PCRP-PCGF6-1A2

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SKU: PCRP-PCGF6-1A2

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DSHB Data Sheet

Catalog Fields

Product Name/ID: PCRP-PCGF6-1A2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: PCGF6
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2618940 
Uniprot ID: Q9BYE7 
RRID:  
Entrez Gene ID: 84108 
Clonality: Monoclonal
Immunogen: Recombinant peptide
Clone:
Immunogen Sequence: Protein Domain
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: Polycomb group ring finger 6
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/1/2015
Antigen Molecular Weight: 39.05
Depositor: Common Fund – Protein Capture Reagents Program
Antigen Sequence:
Depositor Institution: National Institutes of Health
Antigen Species: Human
Depositor Notes: This antigen is also known as Mel18 and Bmi1-like RING finger.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_2618940
Predicted Species Reactivity: Human, Mouse, Rat
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-PCGF6-1A2 was deposited to the DSHB by Common Fund – Protein Capture Reagents Program (DSHB Hybridoma Product PCRP-PCGF6-1A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

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  • Initial Publication
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