PL-3
Catalog Fields
Antigen: plateins (beta- & gamma-)
Hybridoma Cells Available: Yes
Antigen Species: Euplotes aediculatus
Depositor: Kloetzel, J.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Maryland Baltimore County
Positive Tested Species Reactivity: Euplotes, Oxytricha
Depositors Notes: Specificity note: all Euplotes tested (see J. Protozool. 39, 92-101.); some Oxytricha (distantly related stichotrich ciliate). Functional effects: cytoskeletal (surface support). IHC note: positive for sub-surface alveolar plates.
Antigen Molecular Weight: 72.8 kDa (gene sequence) but 95 and 99 kDa by SDS-PAGE
Predicted Species Reactivity:
Gene:
Immunogen: Euplotes aediculatus whole cell homogenate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63AG8.653
Epitope Location or Sequence: Partial seq; see PMID 12615971 & 12674476
Antibody Registry ID: AB_1161639
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PL-3 was deposited to the DSHB by Kloetzel, J.A. (DSHB Hybridoma Product PL-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References