PL-3
Catalog Fields
Clone ID/Product Name: PL-3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_1161639
Clonality: Monoclonal
Immunogen: Euplotes aediculatus whole cell homogenate
Clone:
Immunogen Sequence:
Myeloma Strain: P3X63AG8.653
Epitope Mapped: No
Antigen Name: plateins (beta- & gamma-)
Epitope Location or Sequence: Partial seq; see PMID 12615971 & 12674476
Alternate Antigen Name:
Deposit Date: 5/11/2007
Antigen Molecular Weight: 72.8 kDa (gene sequence) but 95 and 99 kDa by SDS-PAGE
Depositor: Kloetzel, J.A.
Antigen Sequence:
Depositor Institution: University of Maryland Baltimore County
Antigen Species: Euplotes aediculatus
Depositor Notes: Specificity note: all Euplotes tested (see J. Protozool. 39, 92-101.); some Oxytricha (distantly related stichotrich ciliate). Functional effects: cytoskeletal (surface support). IHC note: positive for sub-surface alveolar plates.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Euplotes, Oxytricha
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PL-3 was deposited to the DSHB by Kloetzel, J.A. (DSHB Hybridoma Product PL-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
2 References