R5A

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$40.00
SKU: R5A
View product citations for antibody R5A on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Reelin
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Goffinet, A.
Isotype: MIgG2b
Depositors Institution: University of Louvain, Belgium
Host Species: mouse
Depositors Notes: R5A was generated in Reln-Orl mutant mice.
Positive Tested Species Reactivity: Human, Mouse, Rodent
Gene: Reln
Antigen Molecular Weight: 387.5 kDa
Alternate Gene Names: Rl
Immunogen: Reelin protein domain a.a. 1796-2115
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: Q60841 
Epitope Location or Sequence: Central region a.a. 1925-2058
Entrez Gene ID: 19699 
Immunogen Sequence: Synthetic peptide-BSA conjugate
Antibody Registry ID: AB_2179314 
Additional Information: RRID:AB_2179314
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R5A was deposited to the DSHB by Goffinet, A. (DSHB Hybridoma Product R5A)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    IF References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    WB References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Functional importance of covalent homodimer of reelin protein linked via its central region.
    Takagi J
    The Journal of biological chemistry 286.40 (2011 Oct 7): 35247-56.

    IHC References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    IP References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    ELISA References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    FFPE References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    FB References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Epitope Map References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    All References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Functional importance of covalent homodimer of reelin protein linked via its central region.
    Takagi J
    The Journal of biological chemistry 286.40 (2011 Oct 7): 35247-56.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

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