Ralph 3.1(original clone 6B3)

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SKU: Ralph 3.1(original clone 6B3)
View product citations for antibody Ralph 3.1(original clone 6B3) on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Ralph 3.1(original clone 6B3)
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: Itga3
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2234085 
Uniprot ID: D3ZCG9 
Entrez Gene ID: 360606 
Clonality: Monoclonal
Immunogen: purified Rugli cell-derived rat integrin alpha 3
Immunogen Sequence: Full length protein sequence
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: integrin alpha-3
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/20/1999
Antigen Molecular Weight:
Depositor: Reichardt, L.
Antigen Sequence:
Depositor Institution: University of California, San Francisco
Antigen Species: rat
Depositor Notes: Cell binding note: extracellular domain of rat integrin alpha 3.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information: Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : ita@ucsf.edu Telephone: 415 502-1603.

Ralph 3.1 blocks adhesion to collagens I and IV. See PMID: 16269008.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ralph 3.1(original clone 6B3) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product Ralph 3.1(original clone 6B3))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IHC References
    IP References
    FB References
    All References

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