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Ralph 3.1(original clone 6B3)

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$40.00
SKU: Ralph 3.1(original clone 6B3)
View product citations for antibody Ralph 3.1(original clone 6B3) on CiteAb

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Available: 23

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-3
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Reichardt, L.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, San Francisco
Positive Tested Species Reactivity: Rat
Depositors Notes: Cell binding note: extracellular domain of rat integrin alpha 3.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: purified Rugli cell-derived rat integrin alpha 3
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ralph 3.1(original clone 6B3) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product Ralph 3.1(original clone 6B3))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IHC References
    IP References
    All References

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