Rop 4F8

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$40.00
SKU: Rop 4F8
View product citations for antibody Rop 4F8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Rop Protein/ Ras opposite protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 67.8 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Rop
Immunogen: Recombinant GST-Rop fusion protein
Alternate Gene Names: Dmel_CG15811
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q07327 
Immunogen Sequence: a.a. 180-389
Entrez Gene ID: 38493 
Additional Characterization:  
Antibody Registry ID: AB_1157869 
Additional Information: RRID:AB_1157869. FBgn0004574.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rop 4F8 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product Rop 4F8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    All References

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