SA-DA4

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SKU: SA-DA4-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: SA-DA4
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: IGHM
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2796572 
Uniprot ID: P01871 
RRID:  
Entrez Gene ID: 3507 
Clonality: Monoclonal
Immunogen: IgM myeloma protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: P3X63.Ag8.653
Epitope Mapped: No
Antigen Name: Human IgM
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 51.9 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Human
Depositor Notes: Hybridoma developed by J.F.Kearney and M.D. Cooper.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SA-DA4 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product SA-DA4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 135.1 (1985 Jul): 192-9.

    IF References
    WB References
    IHC References
    IP References

    Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells.
    Kubagawa H
    Journal of immunology (Baltimore, Md. : 1950) 138.12 (1987 Jun 15): 4150-8.

    Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies.
    Cooper MD
    Proceedings of the National Academy of Sciences of the United States of America 89.18 (1992 Sep 15): 8522-6.

    ELISA References

    Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 135.1 (1985 Jul): 192-9.

    Mouse/human chimeric antibodies to a tumor-associated antigen: biologic activity of the four human IgG subclasses.
    LoBuglio AF
    Journal of the National Cancer Institute 80.19 (1988 Dec 7): 1553-9.

    FB References
    FACS References

    Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies.
    Cooper MD
    Journal of immunology (Baltimore, Md. : 1950) 135.1 (1985 Jul): 192-9.

    Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies.
    Cooper MD
    Proceedings of the National Academy of Sciences of the United States of America 89.18 (1992 Sep 15): 8522-6.

    Immunoglobulin D enhances immune surveillance by activating antimicrobial, proinflammatory and B cell-stimulating programs in basophils.
    Cerutti A
    Nature immunology 10.8 (2009 Aug): 889-98.

    All References

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