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View product citations for antibody SMED 2E8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Pigment cups
Hybridoma Cells Available: Yes
Antigen Species: Schmidtea mediterranea/Planaria
Depositor: Newmark, P.A. / Forsthoefel, D.J. / Waters, F.A.
Isotype: MIgM, kappa light chain
Depositors Institution: University of Illinois
Host Species: mouse
Depositors Notes: See validation citation for fixation optimization.
Positive Tested Species Reactivity: Planaria, Schmidtea mediterranea
Clonality: Monoclonal
Immunogen: phagocytes, gastrodermal (cell line C1W4) fixed
Additional Information: RRID:AB_2722477
Myeloma Strain: Sp 2/0
Antibody Registry ID: AB_2722477 
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SMED 2E8 was deposited to the DSHB by Newmark, P.A. / Forsthoefel, D.J. / Waters, F.A. (DSHB Hybridoma Product SMED 2E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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