Sp-25

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SKU: Sp-25

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Sp-25
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Sp-EGF I, Sp-EGF III
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2889818 
Uniprot ID: W4XAL5 
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Extracted Apical Lamina proteins
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Sp-EGF I, Sp-EGF III
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/22/2017
Antigen Molecular Weight: 180 kDa, 140 kDa
Depositor: Burke, R.D.
Antigen Sequence:
Depositor Institution: University of Victoria, Department of Biochemistry & Microbiology
Antigen Species: S. purpuratus
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: S. purpuratus
Additional Information: RRID: AB_2889818
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Sp-25 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product Sp-25)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication

    Cell movements during the initial phase of gastrulation in the sea urchin embryo.
    Jackson C
    Developmental biology 146.2 (1991 Aug): 542-57.

    IHC References

    Cell movements during the initial phase of gastrulation in the sea urchin embryo.
    Jackson C
    Developmental biology 146.2 (1991 Aug): 542-57.

    All References

    Cell movements during the initial phase of gastrulation in the sea urchin embryo.
    Jackson C
    Developmental biology 146.2 (1991 Aug): 542-57.

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