Antigen: Sp-BetaC ,Sp-BetaCi_1
Hybridoma Cells Available: Yes
Antigen Species: S. purpuratus
Depositor: Burke, R.D.
Host Species: mouse
Depositors Institution: Department of Biochemistry & Microbiology, University of Victoria
Positive Tested Species Reactivity: S. purpuratus
Antigen Molecular Weight: 130 and 225 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: Sp-BetaC ,Sp-BetaCi_1
Immunogen: E. coli expressed recombinant protein fragment
Alternate Gene Names: Spu-020342, Spu-027915
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P91808
Immunogen Sequence: a.a. 141-371
Entrez Gene ID:
Antibody Registry ID:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Sp-2D2 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product Sp-2D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).