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SKU: Sp-2D2
View product citations for antibody Sp-2D2 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Sp-BetaC ,Sp-BetaCi_1
Hybridoma Cells Available: Yes
Antigen Species: S. purpuratus
Depositor: Burke, R.D.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Biochemistry & Microbiology, University of Victoria
Positive Tested Species Reactivity: S. purpuratus
Depositors Notes:
Antigen Molecular Weight: 130 and 225 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Sp-BetaC ,Sp-BetaCi_1
Immunogen: E. coli expressed recombinant protein fragment
Alternate Gene Names: Spu-020342, Spu-027915
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P91808 
Immunogen Sequence: a.a. 141-371
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2753220 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Sp-2D2 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product Sp-2D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    All References

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