Sqd A 8F3

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$45.00 to $425.00
SKU: Sqd A 8F3
View product citations for antibody Sqd A 8F3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Squid protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schupbach, T.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: recognizes protein in tissue and on Westerns, recognizes all isoforms of Squid
Antigen Molecular Weight: Multiple isoforms; Predicted: 18, 33, 35, and 36 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: sqd
Immunogen: Recombinant SqdA (= isoform A)/GST fusion protein
Alternate Gene Names: CG16901, hrp40, l(3)j4B4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q08473 
Immunogen Sequence: Full length protein
Entrez Gene ID: 41666 
Additional Characterization:  
Antibody Registry ID: AB_10586641 
Additional Information: Squid is a member of the hnRNPA family of RNA binding proteins
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Sqd A 8F3 was deposited to the DSHB by Schupbach, T. (DSHB Hybridoma Product Sqd A 8F3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • WB References
  • IP References
  • All References
  • Initial Publication
    WB References
    IP References

    Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis.
    Schüpbach T
    Development (Cambridge, England) 131.9 (2004 May): 1949-58.

    Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila.
    Schüpbach T
    Developmental biology 313.2 (2008 Jan 15): 713-24.

    Glorund interactions in the regulation of gurken and oskar mRNAs.
    Gavis ER
    Developmental biology 326.1 (2009 Feb 1): 68-74.

    All References

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