TC31-27F11.C2

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$40.00
SKU: TC31-27F11.C2
View product citations for antibody TC31-27F11.C2 on CiteAb

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Available: 31

DSHB Data Sheet

Catalog Fields

Antigen: Hexon protein, human adenovirus 5
Hybridoma Cells Available: Yes
Antigen Species: human adenovirus C serotype 5
Depositor: Fayadat-Dilman, L. / Olijve, W.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Schering Plough Biopharma
Positive Tested Species Reactivity: human adenovirus 5
Depositors Notes:
Antigen Molecular Weight: Predicted: 108 kDa; Apparent: 108 kDa (monomer, heat-denatured); > 200kDa (trimer, not heat-denatured)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: L3
Immunogen: Purified human adenovirus serotype 5
Alternate Gene Names:
Alternate Antibody Name: 27F11
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag
Epitope Location or Sequence:
Uniprot ID: P04133 
Immunogen Sequence: full length protein
Entrez Gene ID: 2652998 
Additional Characterization:  
Antibody Registry ID: AB_1553403 
Additional Information: Hexon is the major capsid protein. The epitope recognized by TC31-27F11.C2 is likely conformation-specific since this mAb can not bind heat denatured hexon. TC31-27F11.C2 immunoprecipitates only free hexon protein; in contrast, TC31-9C12.C9 (a mAb available in the DSHB collection, isolated from the same fusion that generated TC31-27F11.C2) pulls down the complete set of proteins normally associated with intact Ad5 particles. The TC31-27F11.C2 epitope is likely not accessible on the exposed surface of intact Ad particles. While TC31-9C12.C9 neutralizes Ad5, TC31-27F11.C2 doesn't. TC31-27F11.C2 is the perfect isotype-matched nonneutralizing control Ab for TC31-9C12.C9.
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TC31-27F11.C2 was deposited to the DSHB by Fayadat-Dilman, L. / Olijve, W. (DSHB Hybridoma Product TC31-27F11.C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • WB References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    WB References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    IP References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    ELISA References

    Postentry neutralization of adenovirus type 5 by an antihexon antibody.
    Ralston R
    Journal of virology 78.22 (2004 Nov): 12320-32.

    All References

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