TRA-1-85

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$45.00
SKU: TRA-1-85-s
View product citations for antibody TRA-1-85 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: chromosome 19, OKa blood antigen / CD147
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1
Depositor: Andrews, P.W.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: University of Sheffield
Antigen Molecular Weight: 35-69 kDa
Depositors Notes: The TRA-1-85 monoclonal antibody is often cited as TRA1-85.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: 2120Ep human embryonal carcinoma cells
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/O
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_531824 
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TRA-1-85 was deposited to the DSHB by Andrews, P.W. (DSHB Hybridoma Product TRA-1-85)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • IHC References
  • FACS References
  • All References
  • Initial Publication

    Biochemical and genetic analysis of the OKa blood group antigen.
    Goodfellow PN
    Immunogenetics 27.5 (1988): 322-9.

    IF References

    Von Willebrand factor inhibits mature smooth muscle gene expression through impairment of Notch signaling.
    Wang MM
    PloS one 8.9 (2013): e75808.

    Stromal LRP1 in lung adenocarcinoma predicts clinical outcome.
    Wang MM
    Clinical cancer research : an official journal of the American Association for Cancer Research 17.8 (2011 Apr 15): 2426-33.

    Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Auerbach JM
    Stem cells (Dayton, Ohio) 25.2 (2007 Feb): 437-46.

    Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors.
    Krause KH
    Stem cells (Dayton, Ohio) 24.3 (2006 Mar): 615-23.

    IHC References

    Efficacy and Safety of Human Retinal Progenitor Cells.
    Vugler A
    Translational vision science & technology 5.4 (2016 Jul): 6.

    Localization of blood proteins thrombospondin1 and ADAMTS13 to cerebral corpora amylacea.
    Wang MM
    Neuropathology : official journal of the Japanese Society of Neuropathology 29.6 (2009 Dec): 664-71.

    Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes.
    Sheng HZ
    Cell research 13.4 (2003 Aug): 251-63.

    FACS References
    All References

    Efficacy and Safety of Human Retinal Progenitor Cells.
    Vugler A
    Translational vision science & technology 5.4 (2016 Jul): 6.

    Localization of blood proteins thrombospondin1 and ADAMTS13 to cerebral corpora amylacea.
    Wang MM
    Neuropathology : official journal of the Japanese Society of Neuropathology 29.6 (2009 Dec): 664-71.

    Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes.
    Sheng HZ
    Cell research 13.4 (2003 Aug): 251-63.

    Von Willebrand factor inhibits mature smooth muscle gene expression through impairment of Notch signaling.
    Wang MM
    PloS one 8.9 (2013): e75808.

    Stromal LRP1 in lung adenocarcinoma predicts clinical outcome.
    Wang MM
    Clinical cancer research : an official journal of the American Association for Cancer Research 17.8 (2011 Apr 15): 2426-33.

    Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Auerbach JM
    Stem cells (Dayton, Ohio) 25.2 (2007 Feb): 437-46.

    Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors.
    Krause KH
    Stem cells (Dayton, Ohio) 24.3 (2006 Mar): 615-23.

    Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells.
    Bouhassira EE
    Stem cells (Dayton, Ohio) 24.8 (2006 Aug): 1914-22.

    Biochemical and genetic analysis of the OKa blood group antigen.
    Goodfellow PN
    Immunogenetics 27.5 (1988): 322-9.

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