TRA-1-85

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$40.00
SKU: TRA-1-85-s
View product citations for antibody TRA-1-85 on CiteAb

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Available: 173

DSHB Data Sheet

Catalog Fields

Antigen: chromosome 19, OKa blood antigen / CD147
Hybridoma Cells Available: No
Antigen Species: human
Depositor: Andrews, P.W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Sheffield
Positive Tested Species Reactivity: Human
Depositors Notes: The TRA-1-85 monoclonal antibody is often cited as TRA1-85.
Antigen Molecular Weight: 35-69 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: 2120Ep human embryonal carcinoma cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/O
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531824 
Additional Information:
Recommended Applications: Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TRA-1-85 was deposited to the DSHB by Andrews, P.W. (DSHB Hybridoma Product TRA-1-85)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • IHC References
  • FACS References
  • All References
  • Initial Publication

    Biochemical and genetic analysis of the OKa blood group antigen.
    Goodfellow PN
    Immunogenetics 27.5 (1988): 322-9.

    IF References

    Von Willebrand factor inhibits mature smooth muscle gene expression through impairment of Notch signaling.
    Wang MM
    PloS one 8.9 (2013): e75808.

    Stromal LRP1 in lung adenocarcinoma predicts clinical outcome.
    Wang MM
    Clinical cancer research : an official journal of the American Association for Cancer Research 17.8 (2011 Apr 15): 2426-33.

    Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Auerbach JM
    Stem cells (Dayton, Ohio) 25.2 (2007 Feb): 437-46.

    Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors.
    Krause KH
    Stem cells (Dayton, Ohio) 24.3 (2006 Mar): 615-23.

    IHC References

    Efficacy and Safety of Human Retinal Progenitor Cells.
    Vugler A
    Translational vision science & technology 5.4 (2016 Jul): 6.

    Localization of blood proteins thrombospondin1 and ADAMTS13 to cerebral corpora amylacea.
    Wang MM
    Neuropathology : official journal of the Japanese Society of Neuropathology 29.6 (2009 Dec): 664-71.

    Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes.
    Sheng HZ
    Cell research 13.4 (2003 Aug): 251-63.

    FACS References
    All References

    Efficacy and Safety of Human Retinal Progenitor Cells.
    Vugler A
    Translational vision science & technology 5.4 (2016 Jul): 6.

    Localization of blood proteins thrombospondin1 and ADAMTS13 to cerebral corpora amylacea.
    Wang MM
    Neuropathology : official journal of the Japanese Society of Neuropathology 29.6 (2009 Dec): 664-71.

    Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes.
    Sheng HZ
    Cell research 13.4 (2003 Aug): 251-63.

    Von Willebrand factor inhibits mature smooth muscle gene expression through impairment of Notch signaling.
    Wang MM
    PloS one 8.9 (2013): e75808.

    Stromal LRP1 in lung adenocarcinoma predicts clinical outcome.
    Wang MM
    Clinical cancer research : an official journal of the American Association for Cancer Research 17.8 (2011 Apr 15): 2426-33.

    Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Auerbach JM
    Stem cells (Dayton, Ohio) 25.2 (2007 Feb): 437-46.

    Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors.
    Krause KH
    Stem cells (Dayton, Ohio) 24.3 (2006 Mar): 615-23.

    Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells.
    Bouhassira EE
    Stem cells (Dayton, Ohio) 24.8 (2006 Aug): 1914-22.

    Biochemical and genetic analysis of the OKa blood group antigen.
    Goodfellow PN
    Immunogenetics 27.5 (1988): 322-9.

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